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Science 15 July 2005:
Vol. 309. no. 5733, pp. 473 - 476
DOI: 10.1126/science.1110289
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Reports

The Trypanosoma cruzi Proteome

J. A. Atwood, III,1* D. B. Weatherly,2,3* T. A. Minning,2,3 B. Bundy,2,3 C. Cavola,1 F. R. Opperdoes,4 R. Orlando,1 R. L. Tarleton2,3{dagger}

To complement the sequencing of the three kinetoplastid genomes reported in this issue, we have undertaken a whole-organism, proteomic analysis of the four life-cycle stages of Trypanosoma cruzi. Peptides mapping to 2784 proteins in 1168 protein groups from the annotated T. cruzi genome were identified across the four life-cycle stages. Protein products were identified from >1000 genes annotated as "hypothetical" in the sequenced genome, including members of a newly defined gene family annotated as mucin-associated surface proteins. The four parasite stages appear to use distinct energy sources, including histidine for stages present in the insect vectors and fatty acids by intracellular amastigotes.

1 Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA.
2 Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA.
3 Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
4 Research Unit for Tropical Diseases and Laboratory of Biochemistry, Christian de Duve Institute of Cellular Pathology, and Catholic University of Louvain, Brussels, Belgium.

* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: tarleton{at}cb.uga.edu

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