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Bone Marrow Stromal Cells Generate Muscle Cells and Repair Muscle Degeneration
Mari Dezawa,1*Hiroto Ishikawa,1Yutaka Itokazu,1Tomoyuki Yoshihara,1Mikio Hoshino,2Shin-ichi Takeda,3Chizuka Ide,1Yo-ichi Nabeshima2
Bone marrow stromal cells (MSCs) have great potential as therapeuticagents. We report a method for inducing skeletal muscle lineagecells from human and rat general adherent MSCs with an efficiencyof 89%. Induced cells differentiated into muscle fibers upontransplantation into degenerated muscles of rats and mdx-nudemice. The induced population contained Pax7-positive cells thatcontributed to subsequent regeneration of muscle upon repetitivedamage without additional transplantation of cells. These MSCsrepresent a more ready supply of myogenic cells than do therare myogenic stem cells normally found in muscle and bone marrow.
1 Department of Anatomy and Neurobiology, Kyoto University Graduate School of Medicine, Yoshidakonoecho, Sakyo-ku, Kyoto, 606-8501 Japan. 2 Department of Pathology and Tumor Biology, Kyoto University Graduate School of Medicine, Yoshidakonoecho, Sakyo-ku, Kyoto, 606-8501 Japan. 3 Department of Molecular Therapy, National Center of Neurology and Psychiatry, Kodaira, 187-8502 Tokyo, Japan.
* To whom correspondence should be addressed. E-mail: dezawa{at}anat2.med.kyoto-u.ac.jp
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R. Atoui, D. Shum-Tim, and R. C.J. Chiu (2008)
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R. Siddappa, A. Martens, J. Doorn, A. Leusink, C. Olivo, R. Licht, L. van Rijn, C. Gaspar, R. Fodde, F. Janssen, et al. (2008)
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Discordant proliferation and differentiation in pituitary tumor-transforming gene-null bone marrow stem cells.
T. Rubinek, V. Chesnokova, I. Wolf, K. Wawrowsky, G. Vlotides, and S. Melmed (2007)
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293, C1082-C1092
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S. H.A. Wong, K. N. Lowes, I. Bertoncello, A. F. Quigley, P. J. Simmons, M. J. Cook, A. J. Kornberg, and R. M.I. Kapsa (2007)
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Menstrual Blood-derived Cells Confer Human Dystrophin Expression in the Murine Model of Duchenne Muscular Dystrophy via Cell Fusion and Myogenic Transdifferentiation.
C.-H. Cui, T. Uyama, K. Miyado, M. Terai, S. Kyo, T. Kiyono, and A. Umezawa (2007)
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Widespread Distribution and Muscle Differentiation of Human Fetal Mesenchymal Stem Cells After Intrauterine Transplantation in Dystrophic mdx Mouse.
J. Chan, S. N. Waddington, K. O'Donoghue, H. Kurata, P. V. Guillot, C. Gotherstrom, M. Themis, J. E. Morgan, and N. M. Fisk (2007)
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J. Chan, K. O'Donoghue, M. Gavina, Y. Torrente, N. Kennea, H. Mehmet, H. Stewart, D. J. Watt, J. E. Morgan, and N. M. Fisk (2006)
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Myogenic potential of adipose-tissue-derived cells.
G. Di Rocco, M. G. Iachininoto, A. Tritarelli, S. Straino, A. Zacheo, A. Germani, F. Crea, and M. C. Capogrossi (2006)
J. Cell Sci.
119, 2945-2952
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T. Partridge (2006)
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