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Science 8 July 2005:
Vol. 309. no. 5732, pp. 297 - 300
DOI: 10.1126/science.1113465
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Reports

The Kinesin Klp2 Mediates Polarization of Interphase Microtubules in Fission Yeast

Rafael E. Carazo-Salas,1* Claude Antony,2 Paul Nurse1,3

Fission yeast (Schizosaccharomyces pombe) cells grow longitudinally in a manner dependent on a polarized distribution of their interphase microtubules. We found that this distribution required sliding of microtubules toward the cell center along preexisting microtubules. This sliding was mediated by the minus end–directed kinesin motor Klp2, which helped microtubules to become properly organized with plus ends predominantly oriented toward the cell ends and minus ends toward the cell center. Thus, interphase microtubules in the fission yeast require motor activities for their proper organization.

1 Cell Cycle Laboratory, 44 Lincoln's Inn Fields, Cancer Research UK, London Research Institute, WC2A 1PX, UK.
2 European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
3 Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.

* To whom correspondence should be addressed. E-mail: carazo01{at}cancer.org.uk

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Diffusion and Directed Movement: IN VITRO MOTILE PROPERTIES OF FISSION YEAST KINESIN-14 Pkl1.
K. Furuta, M. Edamatsu, Y. Maeda, and Y. Y. Toyoshima (2008)
J. Biol. Chem. 283, 36465-36473
   Abstract »    Full Text »    PDF »
Stress-regulated kinase pathways in the recovery of tip growth and microtubule dynamics following osmotic stress in S. pombe.
A. M. Robertson and I. M. Hagan (2008)
J. Cell Sci. 121, 4055-4068
   Abstract »    Full Text »    PDF »
Mitotic Chromosome Biorientation in Fission Yeast Is Enhanced by Dynein and a Minus-end-directed, Kinesin-like Protein.
E. L. Grishchuk, I. S. Spiridonov, and J. R. McIntosh (2007)
Mol. Biol. Cell 18, 2216-2225
   Abstract »    Full Text »    PDF »
Hyphal Growth: a Tale of Motors, Lipids, and the Spitzenkorper.
G. Steinberg (2007)
Eukaryot. Cell 6, 351-360
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Generation of noncentrosomal microtubule arrays.
F. Bartolini and G. G. Gundersen (2006)
J. Cell Sci. 119, 4155-4163
   Abstract »    Full Text »    PDF »
S. pombe CLASP needs dynein, not EB1 or CLIP170, to induce microtubule instability and slows polymerization rates at cell tips in a dynein-dependent manner.
A. Grallert, C. Beuter, R. A. Craven, S. Bagley, D. Wilks, U. Fleig, and I. M. Hagan (2006)
Genes & Dev. 20, 2421-2436
   Abstract »    Full Text »    PDF »
Dynein-dependent Motility of Microtubules and Nucleation Sites Supports Polarization of the Tubulin Array in the Fungus Ustilago maydis.
G. Fink and G. Steinberg (2006)
Mol. Biol. Cell 17, 3242-3253
   Abstract »    Full Text »    PDF »
The Fission Yeast Transforming Acidic Coiled Coil-related Protein Mia1p/Alp7p Is Required for Formation and Maintenance of Persistent Microtubule-organizing Centers at the Nuclear Envelope.
L. Zheng, C. Schwartz, L. Wee, and S. Oliferenko (2006)
Mol. Biol. Cell 17, 2212-2222
   Abstract »    Full Text »    PDF »


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