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Originally published in Science Express on 7 April 2005
Science 3 June 2005:
Vol. 308. no. 5727, pp. 1469 - 1472
DOI: 10.1126/science.1108408
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Reports

Kinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement?

Comert Kural,1 Hwajin Kim,3 Sheyum Syed,2 Gohta Goshima,4 Vladimir I. Gelfand,3*{dagger} Paul R. Selvin1,2{ddagger}

We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)–tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be ~8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.

1 Biophysics Center, University of Illinois, Urbana, IL 61801, USA.
2 Physics Department, University of Illinois, Urbana, IL 61801, USA.
3 Department of Cell and Structural Biology, University of Illinois, Urbana, IL 61801, USA.
4 Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94107, USA.

* Present address: Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

{dagger} To whom correspondence should be addressed. Department of Cell and Molecular Biology, North-western University School of Medicine, 303 East Chicago Avenue, Ward 11-080, Chicago, IL 60611–3008, USA. E-mail: vgelfand{at}northwestern.edu {ddagger}To whom correspondence should be addressed. Loomis Lab of Physics, 1110 West Green Street, University of Illinois, Urbana, IL 61801, USA. E-mail: selvin{at}uiuc.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)