Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 8 April 2005:
Vol. 308. no. 5719, pp. 227 - 231
DOI: 10.1126/science.1106885
Prev | Table of Contents | Next

Research Articles

Structure of a {gamma}{delta} T Cell Receptor in Complex with the Nonclassical MHC T22

Erin J. Adams,1,2,3 Yueh-Hsiu Chien,1,3 K. Christopher Garcia1,2,3*

{gamma}{delta} T cell receptors (TCRs), {alpha}ß TCRs, and antibodies are the three lineages of somatically recombined antigen receptors. The structural basis for ligand recognition is well defined for {alpha}ß TCR and antibodies but is lacking for {gamma}{delta} TCRs. We present the 3.4 Å structure of the murine {gamma}{delta} TCR G8 bound to its major histocompatibility complex (MHC) class Ib ligand, T22. G8 predominantly uses germline-encoded residues of its {delta} chain complementarity-determining region 3 (CDR3) loop to bind T22 in an orientation substantially different from that seen in {alpha}ß TCR/peptide-MHC. That junctionally encoded G8 residues play an ancillary role in binding suggests a fusion of innate and adaptive recognition strategies.

1 Department of Microbiology and Immunology
2 Department of Structural Biology
3 Program in Immunology, Stanford University School of Medicine, Fairchild D319, 299 Campus Drive, Stanford, CA 94035–5124, USA.

* To whom correspondence should be addressed. E-mail: kcgarcia{at}stanford.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
{gamma}{delta} T Lymphocyte Homeostasis Is Negatively Regulated by {beta}2-Microglobulin.
J. D. French, C. L. Roark, W. K. Born, and R. L. O'Brien (2009)
J. Immunol. 182, 1892-1900
   Abstract »    Full Text »    PDF »
Photoaffinity Antigens for Human {gamma}{delta} T Cells.
G. Sarikonda, H. Wang, K.-J. Puan, X.-h. Liu, H. K. Lee, Y. Song, M. D. Distefano, E. Oldfield, G. D. Prestwich, and C. T. Morita (2008)
J. Immunol. 181, 7738-7750
   Abstract »    Full Text »    PDF »
Identification of Human T Cell Receptor {gamma}{delta}-recognized Epitopes/Proteins via CDR3{delta} Peptide-based Immunobiochemical Strategy.
H. Chen, X. He, Z. Wang, D. Wu, H. Zhang, C. Xu, H. He, L. Cui, D. Ba, and W. He (2008)
J. Biol. Chem. 283, 12528-12537
   Abstract »    Full Text »    PDF »
Bovine Luteal Cells Stimulate Proliferation of Major Histocompatibility Nonrestricted Gamma Delta T Cells.
T. L Davis and J. L Pate (2007)
Biol Reprod 77, 914-922
   Abstract »    Full Text »    PDF »
Mutations Designed to Destabilize the Receptor-Bound Conformation Increase MICA-NKG2D Association Rate and Affinity.
C. S. E. Lengyel, L. J. Willis, P. Mann, D. Baker, T. Kortemme, R. K. Strong, and B. J. McFarland (2007)
J. Biol. Chem. 282, 30658-30666
   Abstract »    Full Text »    PDF »
The Structure of the Macrophage Signal Regulatory Protein {alpha} (SIRP{alpha}) Inhibitory Receptor Reveals a Binding Face Reminiscent of That Used by T Cell Receptors.
D. Hatherley, K. Harlos, D. C. Dunlop, D. I. Stuart, and A. N. Barclay (2007)
J. Biol. Chem. 282, 14567-14575
   Abstract »    Full Text »    PDF »
Depletion of {gamma}{delta} T Cells Exacerbates Murine Adriamycin Nephropathy.
H. Wu, Y. M. Wang, Y. Wang, M. Hu, G. Y. Zhang, J. F. Knight, D. C.H. Harris, and S. I. Alexander (2007)
J. Am. Soc. Nephrol. 18, 1180-1189
   Abstract »    Full Text »    PDF »
Comparative gene expression by WC1+ {gamma}{delta} and CD4+ {alpha}{beta} T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1+ {gamma}{delta} T cells..
K. K. Lahmers, J. F. Hedges, M. A. Jutila, M. Deng, M. S. Abrahamsen, and W. C. Brown (2006)
J. Leukoc. Biol. 80, 939-952
   Abstract »    Full Text »    PDF »
The TCR Cbeta FG Loop Regulates {alpha}beta T Cell Development..
M. Touma, H.-C. Chang, T. Sasada, M. Handley, L. K. Clayton, and E. L. Reinherz (2006)
J. Immunol. 176, 6812-6823
   Abstract »    Full Text »    PDF »
Epithelial Cells and Their Neighbors. III. Interactions between intraepithelial lymphocytes and neighboring epithelial cells.
W. L. Havran, J. M. Jameson, and D. A. Witherden (2005)
Am J Physiol Gastrointest Liver Physiol 289, G627-G630
   Abstract »    Full Text »    PDF »
Professional Antigen-Presentation Function by Human {gamma}{delta} T Cells.
M. Brandes, K. Willimann, and B. Moser (2005)
Science 309, 264-268
   Abstract »    Full Text »    PDF »
Antigen Recognition Determinants of {gamma}{delta} T Cell Receptors.
S. Shin, R. El-Diwany, S. Schaffert, E. J. Adams, K. C. Garcia, P. Pereira, and Y.-h. Chien (2005)
Science 308, 252-255
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)