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Science 18 February 2005:
Vol. 307. no. 5712, pp. 1095 - 1098
DOI: 10.1126/science.1108061
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Reports

Golgin Tethers Define Subpopulations of COPI Vesicles

Jörg Malsam,*{dagger} Ayano Satoh,* Laurence Pelletier,{dagger} Graham Warren{ddagger}

Coiled-coil proteins of the golgin family have been implicated in intra-Golgi transport through tethering coat protein complex I (COPI) vesicles. The p115-golgin tether is the best studied, and here we characterize the golgin-84–CASP tether. The vesicles bound by this tether were strikingly different from those bound by the p115-golgin tether in that they lacked members of the p24 family of putative cargo receptors and contained enzymes instead of anterograde cargo. Microinjected golgin-84 or CASP also inhibited Golgi-enzyme transport to the endoplasmic reticulum, further implicating this tether in retrograde transport. These and other golgins may modulate the flow patterns within the Golgi stack.

Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520–8002, USA.

* These authors contributed equally to this work.

{dagger} Present address: Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany.

{ddagger} To whom correspondence should be addressed. E-mail: graham.warren{at}yale.edu

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