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Science 28 January 2005:
Vol. 307. no. 5709, pp. 538 - 544
DOI: 10.1126/science.1104274
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Review

Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics

X. Michalet,1* F. F. Pinaud,1* L. A. Bentolila,1 J. M. Tsay,1 S. Doose,1{dagger} J. J. Li,1 G. Sundaresan,2 A. M. Wu,2 S. S. Gambhir,2,4 S. Weiss1,3*

Research on fluorescent semiconductor nanocrystals (also known as quantum dots or qdots) has evolved over the past two decades from electronic materials science to biological applications. We review current approaches to the synthesis, solubilization, and functionalization of qdots and their applications to cell and animal biology. Recent examples of their experimental use include the observation of diffusion of individual glycine receptors in living neurons and the identification of lymph nodes in live animals by near-infrared emission during surgery. The new generations of qdots have far-reaching potential for the study of intracellular processes at the single-molecule level, high-resolution cellular imaging, long-term in vivo observation of cell trafficking, tumor targeting, and diagnostics.

1 Department of Chemistry and Biochemistry, University of California, 607 Charles E. Young Drive East, Los Angeles, CA 90095, USA.
2 Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, 700 Westwood Plaza, Los Angeles, CA 90095, USA.
3 Department of Physiology, David Geffen School of Medicine, University of California, 700 Westwood Plaza, Los Angeles, CA 90095, USA.
4 Department of Radiology and Bio-X Program, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA 94305, USA.



{dagger} Present address: Angewandte Laserphysik & Laser-specktroskopie, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany.

* To whom correspondence should be addressed. E-mail: michalet{at}chem.ucla.edu (X.M.); fpinaud{at}chem.ucla.edu (F.F.P.); sweiss{at}chem.ucla.edu (S.W.)

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