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Science 21 January 2005:
Vol. 307. no. 5708, pp. 421 - 423
DOI: 10.1126/science.1106478
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Reports

Mechanism of hsp70i Gene Bookmarking

Hongyan Xing,1 Donald C. Wilkerson,1 Christopher N. Mayhew,1,3 Eric J. Lubert,1,4 Hollie S. Skaggs,1 Michael L. Goodson,1,5 Yiling Hong,1,3 Ok-Kyong Park-Sarge,2 Kevin D. Sarge1*

In contrast to most genomic DNA in mitotic cells, the promoter regions of some genes, such as the stress-inducible hsp70i gene that codes for a heat shock protein, remain uncompacted, a phenomenon called bookmarking. Here we show that hsp70i bookmarking is mediated by a transcription factor called HSF2, which binds this promoter in mitotic cells, recruits protein phosphatase 2A, and interacts with the CAP-G subunit of the condensin enzyme to promote efficient dephosphorylation and inactivation of condensin complexes in the vicinity, thereby preventing compaction at this site. Blocking HSF2-mediated bookmarking by HSF2 RNA interference decreases hsp70i induction and survival of stressed cells in the G1 phase, which demonstrates the biological importance of gene bookmarking.

1 Department of Molecular and Cellular Biochemistry, Chandler Medical Center, University of Kentucky, Lexington, KY 40536, USA.
2 Department of Physiology, Chandler Medical Center, University of Kentucky, Lexington, KY 40536, USA.
3 Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati, Cincinnati, OH 45267, USA.
4 Battelle Memorial Institute, Columbus, OH 43201, USA.
5 Section of Microbiology, University of California at Davis, Davis, CA 95616, USA.

* To whom correspondence should be addressed. E-mail: kdsarge{at}uky.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)