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Acetylation by Tip60 Is Required for Selective Histone Variant Exchange at DNA Lesions
Thomas Kusch,1*Laurence Florens,1W. Hayes MacDonald,2Selene K. Swanson,1Robert L. Glaser,3John R. Yates, III,2Susan M. Abmayr,1Michael P Washburn,1Jerry L. Workman1*
Phosphorylation of the human histone variant H2A.X and H2Av,its homolog in Drosophila melanogaster, occurs rapidly at sitesof DNA double-strand breaks. Little is known about the functionof this phosphorylation or its removal during DNA repair. Here,we demonstrate that the Drosophila Tip60 (dTip60) chromatin-remodelingcomplex acetylates nucleosomal phospho-H2Av and exchanges itwith an unmodified H2Av. Both the histone acetyltransferasedTip60 as well as the adenosine triphosphatase Domino/p400 catalyzethe exchange of phospho-H2Av. Thus, these data reveal a previouslyunknown mechanism for selective histone exchange that uses theconcerted action of two distinct chromatin-remodeling enzymeswithin the same multiprotein complex.
1 Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA. 2 Department of Cell Biology, Scripps Research Institute, 10550 North Torrey Pines Road, SR11, La Jolla, CA 92037, USA. 3 Wadsworth Center, New York State Department of Health, Post Office Box 22002, Albany, NY 12201, USA.
* To whom correspondence should be addressed. E-mail: tnk{at}stowers-institute.org (T.K.); jlw{at}stowers-institute.org (J.L.W.)
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