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Originally published in Science Express on 28 October 2004
Science 19 November 2004:
Vol. 306. no. 5700, pp. 1387 - 1390
DOI: 10.1126/science.1104808
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Reports

Nuclear Pore Complex Structure and Dynamics Revealed by Cryoelectron Tomography

Martin Beck, Friedrich Förster, Mary Ecke, Jürgen M. Plitzko, Frauke Melchior,* Günther Gerisch, Wolfgang Baumeister,{dagger} Ohad Medalia{dagger}

Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. Subvolumes of the tomograms containing individual NPCs were extracted in silico and subjected to three-dimensional classification and averaging, whereby distinct structural states were observed. The central plug/transporter (CP/T) was variable in volume and could occupy different positions along the nucleocytoplasmic axis, which supports the notion that it essentially represents cargo in transit. Changes in the position of the CP/T were accompanied by structural rearrangements in the NPC scaffold.

Max Planck Institute of Biochemistry, D-82152 Martinsried, Germany.



* Present address: Department of Biochemistry, University of Göttingen, D-37073 Göttingen, Germany.

{dagger} To whom correspondence should be addressed. E-mail: baumeist{at}biochem.mpg.de (W.B.) and omedalia{at}biochem.mpg.de (O.M.)

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