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Science 17 September 2004:
Vol. 305. no. 5691, pp. 1786 - 1789
DOI: 10.1126/science.1101666
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Reports

Zooming In on a Quantitative Trait for Tomato Yield Using Interspecific Introgressions

Eyal Fridman,1*{dagger} Fernando Carrari,2{dagger} Yong-Sheng Liu,1{ddagger} Alisdair R. Fernie,2 Dani Zamir1§

To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii) in the background of the cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield of tomatoes and was mapped within a flower- and fruit-specific invertase (LIN5). QTL analysis representing five different tomato species delimited the functional polymorphism of Brix9-2-5 to an amino acid near the catalytic site of the invertase crystal, affecting enzyme kinetics and fruit sink strength. These results underline the power of diverse ILs for high-resolution perspectives on complex phenotypes.

1 The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, The Hebrew University of Jerusalem, Post Office Box 12, Rehovot 76100, Israel.
2 Department Willmitzer, Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm, Germany.



* Present address: Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI 48109–1048, USA.

{dagger} These authors contributed equally to this work.

{ddagger} Present address: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.

§ To whom correspondence should be addressed. E-mail: zamir{at}agri.huji.ac.il

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