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Science 10 September 2004:
Vol. 305. no. 5690, pp. 1626 - 1628
DOI: 10.1126/science.1099930
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Reports

Iron-Source Preference of Staphylococcus aureus Infections

Eric P. Skaar,1,2 Munir Humayun,3* Taeok Bae,1,2 Kristin L. DeBord,1,2 Olaf Schneewind1,2{dagger}

Although bacteria use different iron compounds in vitGro, the possibility that microbes distinguish between these iron sources during infection has hitherto not been examined. We applied stable isotope labeling to detect source-specific iron by mass spectrometry and show that Staphylococcus aureus preferentially imports heme iron over transferrin iron. By combining this approach with computational genome analysis, we identified hts (heme transport system), a gene cluster that promotes preferred heme iron import by S. aureus. Heme iron scavenging by means of hts is required for staphylococcal pathogenesis in animal hosts, indicating that heme iron is the preferred iron source during the initiation of infection.

1 Committee on Microbiology, 920 East 58th Street, University of Chicago, Chicago, IL 60637, USA.
2 Department of Molecular Genetics and Cell Biology, 920 East 58th Street, University of Chicago, Chicago, IL 60637, USA.
3 Department of Geophysical Sciences, 5734 South Ellis Avenue, University of Chicago, Chicago, IL 60637, USA.



* Present address: National High Magnetic Field Laboratory, 1800 East Paul Dirac Drive, Florida State University, Tallahassee, FL 32310, USA.

{dagger} To whom correspondence should be addressed. E-mail: oschnee{at}bsd.uchicago.edu

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