Related Content
Search Google Scholar for:
More Information
Related Jobs from ScienceCareers
|
Originally published in Science Express on 29 July 2004
Science 3 September 2004: Vol. 305. no. 5689, pp. 1434 - 1437
DOI: 10.1126/science.1102514
|
|
Research Articles
Crystal Structure of Argonaute and Its Implications for RISC Slicer Activity
Ji-Joon Song,1,2
Stephanie K. Smith,2
Gregory J. Hannon,1
Leemor Joshua-Tor1,2*
Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNA interference effector complex RNA-induced silencing complex (RISC). However, the identity of "Slicer," the enzyme that cleaves the messenger RNA (mRNA) as directed by the siRNA, has not been resolved. Here, we report the crystal structure of the Argonaute protein from Pyrococcus furiosus at 2.25 angstrom resolution. The structure reveals a crescent-shaped base made up of the amino-terminal, middle, and PIWI domains. The Piwi Argonaute Zwille (PAZ) domain is held above the base by a "stalk"-like region. The PIWI domain (named for the protein piwi) is similar to ribonuclease H, with a conserved active site aspartate-aspartate-glutamate motif, strongly implicating Argonaute as "Slicer." The architecture of the molecule and the placement of the PAZ and PIWI domains define a groove for substrate binding and suggest a mechanism for siRNA-guided mRNA cleavage.
1 Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
2 Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
* To whom correspondence should be addressed. E-mail: leemor{at}cshl.edu
Read the Full Text
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- Bax1 Is a Novel Endonuclease: IMPLICATIONS FOR ARCHAEAL NUCLEOTIDE EXCISION REPAIR.
- H. M. Roth, I. Tessmer, B. Van Houten, and C. Kisker (2009)
J. Biol. Chem.
284, 32272-32278
| Abstract »
| Full Text »
| PDF »
- Off-target and a portion of target-specific siRNA mediated mRNA degradation is Ago2 'Slicer' independent and can be mediated by Ago1.
- T. A. Vickers, W. F. Lima, H. Wu, J. G. Nichols, P. S. Linsley, and S. T. Crooke (2009)
Nucleic Acids Res.
37, 6927-6941
| Abstract »
| Full Text »
| PDF »
- Evolution of Biological Catalysis: Ribozyme to RNP Enzyme.
- T.R. Cech (2009)
Cold Spring Harb Symp Quant Biol
| Abstract »
| PDF »
- Structural and biochemical insights into 2'-O-methylation at the 3'-terminal nucleotide of RNA by Hen1.
- C. Mui Chan, C. Zhou, J. S. Brunzelle, and R. H. Huang (2009)
PNAS
106, 17699-17704
| Abstract »
| Full Text »
| PDF »
- Binding and Cleavage Specificities of Human Argonaute2.
- W. F. Lima, H. Wu, J. G. Nichols, H. Sun, H. M. Murray, and S. T. Crooke (2009)
J. Biol. Chem.
284, 26017-26028
| Abstract »
| Full Text »
| PDF »
- Emerging roles of microRNAs as molecular switches in the integrated circuit of the cancer cell.
- G. Sotiropoulou, G. Pampalakis, E. Lianidou, and Z. Mourelatos (2009)
RNA
15, 1443-1461
| Abstract »
| Full Text »
| PDF »
- Functional Dissection of the Human TNRC6 (GW182-Related) Family of Proteins.
- D. Baillat and R. Shiekhattar (2009)
Mol. Cell. Biol.
29, 4144-4155
| Abstract »
| Full Text »
| PDF »
- A Novel Virtual Spectrometry: Visualized Regulatory Motifs on ADM, rPol{beta} and CD83 mRNAs in Human-friendly Manners.
- S. Nakamura (2009)
J. Biochem.
146, 251-261
| Abstract »
| Full Text »
| PDF »
- High Throughput Genome-Wide Survey of Small RNAs from the Parasitic Protists Giardia intestinalis and Trichomonas vaginalis.
- X. Chen, L. J. Collins, P. J. Biggs, and D. Penny (2009)
Gen Biol Evol
2009, 165-175
| Abstract »
| Full Text »
| PDF »
- The RNA-induced Silencing Complex: A Versatile Gene-silencing Machine.
- A. J. Pratt and I. J. MacRae (2009)
J. Biol. Chem.
284, 17897-17901
| Abstract »
| Full Text »
| PDF »
- Characterization of the miRNA-RISC loading complex and miRNA-RISC formed in the Drosophila miRNA pathway.
- K. Miyoshi, T. N. Okada, H. Siomi, and M. C. Siomi (2009)
RNA
15, 1282-1291
| Abstract »
| Full Text »
| PDF »
- Small RNA Silencing Pathways in Germ and Stem Cells.
- A.A. Aravin and G.J. Hannon (2009)
Cold Spring Harb Symp Quant Biol
| Abstract »
| PDF »
- Essential and overlapping functions for mammalian Argonautes in microRNA silencing.
- H. Su, M. I. Trombly, J. Chen, and X. Wang (2009)
Genes & Dev.
23, 304-317
| Abstract »
| Full Text »
| PDF »
- Argonaute-2 Expression Is Regulated by Epidermal Growth Factor Receptor and Mitogen-Activated Protein Kinase Signaling and Correlates with a Transformed Phenotype in Breast Cancer Cells.
- B. D. Adams, K. P. Claffey, and B. A. White (2009)
Endocrinology
150, 14-23
| Abstract »
| Full Text »
| PDF »
- RNAi Experiments in Mouse Oocytes and Early Embryos.
- P. Svoboda and P. Stein (2009)
CSH Protocols
2009, pdb.top56
| Abstract »
| Full Text »
- Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs.
- M. Landthaler, D. Gaidatzis, A. Rothballer, P. Y. Chen, S. J. Soll, L. Dinic, T. Ojo, M. Hafner, M. Zavolan, and T. Tuschl (2008)
RNA
14, 2580-2596
| Abstract »
| Full Text »
| PDF »
- Crystal structure of the {beta}-finger domain of Prp8 reveals analogy to ribosomal proteins.
- K. Yang, L. Zhang, T. Xu, A. Heroux, and R. Zhao (2008)
PNAS
105, 13817-13822
| Abstract »
| Full Text »
| PDF »
- Effects of local mRNA structure on posttranscriptional gene silencing.
- S. I. Rudnick, J. Swaminathan, M. Sumaroka, S. Liebhaber, and A. M. Gewirtz (2008)
PNAS
105, 13787-13792
| Abstract »
| Full Text »
| PDF »
- p100 increases AT1R expression through interaction with AT1R 3'-UTR.
- K. Paukku, N. Kalkkinen, O. Silvennoinen, K. K. Kontula, and J. Y. A. Lehtonen (2008)
Nucleic Acids Res.
36, 4474-4487
| Abstract »
| Full Text »
| PDF »
- An Argonaute Transports siRNAs from the Cytoplasm to the Nucleus.
- S. Guang, A. F. Bochner, D. M. Pavelec, K. B. Burkhart, S. Harding, J. Lachowiec, and S. Kennedy (2008)
Science
321, 537-541
| Abstract »
| Full Text »
| PDF »
- A multifunctional human Argonaute2-specific monoclonal antibody.
- S. Rudel, A. Flatley, L. Weinmann, E. Kremmer, and G. Meister (2008)
RNA
14, 1244-1253
| Abstract »
| Full Text »
| PDF »
- The PIWI proteins SMEDWI-2 and SMEDWI-3 are required for stem cell function and piRNA expression in planarians.
- D. Palakodeti, M. Smielewska, Y.-C. Lu, G. W. Yeo, and B. R. Graveley (2008)
RNA
14, 1174-1186
| Abstract »
| Full Text »
| PDF »
- Fundamental differences in the equilibrium considerations for siRNA and antisense oligodeoxynucleotide design.
- Z. J. Lu and D. H. Mathews (2008)
Nucleic Acids Res.
36, 3738-3745
| Abstract »
| Full Text »
| PDF »
- Artificial trans-Acting siRNAs Confer Consistent and Effective Gene Silencing.
- M. de la Luz Gutierrez-Nava, M. J. Aukerman, H. Sakai, S. V. Tingey, and R. W. Williams (2008)
Plant Physiology
147, 543-551
| Abstract »
| Full Text »
| PDF »
- Widespread Translational Inhibition by Plant miRNAs and siRNAs.
- P. Brodersen, L. Sakvarelidze-Achard, M. Bruun-Rasmussen, P. Dunoyer, Y. Y. Yamamoto, L. Sieburth, and O. Voinnet (2008)
Science
320, 1185-1190
| Abstract »
| Full Text »
| PDF »
- Diversification of the Core RNA Interference Machinery in Chlamydomonas reinhardtii and the Role of DCL1 in Transposon Silencing.
- J. A. Casas-Mollano, J. Rohr, E.-J. Kim, E. Balassa, K. van Dijk, and H. Cerutti (2008)
Genetics
179, 69-81
| Abstract »
| Full Text »
| PDF »
- Small RNA guides for de novo DNA methylation in mammalian germ cells.
- A. A. Aravin and D. Bourc'his (2008)
Genes & Dev.
22, 970-975
| Abstract »
| Full Text »
| PDF »
- Chp1-Tas3 Interaction Is Required To Recruit RITS to Fission Yeast Centromeres and for Maintenance of Centromeric Heterochromatin.
- J. L. DeBeauchamp, A. Moses, V. J. P. Noffsinger, D. L. Ulrich, G. Job, A. M. Kosinski, and J. F. Partridge (2008)
Mol. Cell. Biol.
28, 2154-2166
| Abstract »
| Full Text »
| PDF »
- Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect.
- K. Ui-Tei, Y. Naito, S. Zenno, K. Nishi, K. Yamato, F. Takahashi, A. Juni, and K. Saigo (2008)
Nucleic Acids Res.
36, 2136-2151
| Abstract »
| Full Text »
| PDF »
- aubergine Gene Overexpression in Somatic Tissues of auberginesting Mutants Interferes With the RNAi Pathway of a yellow Hairpin dsRNA in Drosophila melanogaster.
- V. Specchia, C. Benna, G. M. Mazzotta, A. Piccin, M. A. Zordan, R. Costa, and M. P. Bozzetti (2008)
Genetics
178, 1271-1282
| Abstract »
| Full Text »
| PDF »
- Strand-specific 5'-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity.
- P. Y. Chen, L. Weinmann, D. Gaidatzis, Y. Pei, M. Zavolan, T. Tuschl, and G. Meister (2008)
RNA
14, 263-274
| Abstract »
| Full Text »
| PDF »
- RNA Silencing Gene Truncation in the Filamentous Fungus Aspergillus nidulans.
- T. M. Hammond, J. W. Bok, M. D. Andrewski, Y. Reyes-Dominguez, C. Scazzocchio, and N. P. Keller (2008)
Eukaryot. Cell
7, 339-349
| Abstract »
| Full Text »
| PDF »
- Dicer-1, but not Loquacious, is critical for assembly of miRNA-induced silencing complexes.
- X. Liu, J. K. Park, F. Jiang, Y. Liu, D. McKearin, and Q. Liu (2007)
RNA
13, 2324-2329
| Abstract »
| Full Text »
| PDF »
- DNA Branch Nuclease Activity of Vaccinia A22 Resolvase.
- M. J. Culyba, N. Minkah, Y. Hwang, O.-M. J. Benhamou, and F. D. Bushman (2007)
J. Biol. Chem.
282, 34644-34652
| Abstract »
| Full Text »
| PDF »
- ARGONAUTE4 Is Required for Resistance to Pseudomonas syringae in Arabidopsis.
- A. Agorio and P. Vera (2007)
PLANT CELL
19, 3778-3790
| Abstract »
| Full Text »
| PDF »
- Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme.
- F. Li, P. S. Pallan, M. A. Maier, K. G. Rajeev, S. L. Mathieu, C. Kreutz, Y. Fan, J. Sanghvi, R. Micura, E. Rozners, et al. (2007)
Nucleic Acids Res.
35, 6424-6438
| Abstract »
| Full Text »
| PDF »
- Reduced levels of Ago2 expression result in increased siRNA competition in mammalian cells.
- T. A. Vickers, W. F. Lima, J. G. Nichols, and S. T. Crooke (2007)
Nucleic Acids Res.
35, 6598-6610
| Abstract »
| Full Text »
| PDF »
- Jidong Liu: Probing P-bodies for the secrets of silencing.
- R. Williams (2007)
J. Cell Biol.
178, 894-895
| Abstract »
| Full Text »
| PDF »
- A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway.
- D. O'Carroll, I. Mecklenbrauker, P. P. Das, A. Santana, U. Koenig, A. J. Enright, E. A. Miska, and A. Tarakhovsky (2007)
Genes & Dev.
21, 1999-2004
| Abstract »
| Full Text »
| PDF »
- Combinatorial delivery of small interfering RNAs reduces RNAi efficacy by selective incorporation into RISC.
- D. Castanotto, K. Sakurai, R. Lingeman, H. Li, L. Shively, L. Aagaard, H. Soifer, A. Gatignol, A. Riggs, and J. J. Rossi (2007)
Nucleic Acids Res.
35, 5154-5164
| Abstract »
| Full Text »
| PDF »
- A Double-Stranded-RNA Response Program Important for RNA Interference Efficiency.
- S. Choudhary, H.-C. Lee, M. Maiti, Q. He, P. Cheng, Q. Liu, and Y. Liu (2007)
Mol. Cell. Biol.
27, 3995-4005
| Abstract »
| Full Text »
| PDF »
- Structure of Aquifex aeolicus Argonaute Highlights Conformational Flexibility of the PAZ Domain as a Potential Regulator of RNA-induced Silencing Complex Function.
- U. J. Rashid, D. Paterok, A. Koglin, H. Gohlke, J. Piehler, and J. C.-H. Chen (2007)
J. Biol. Chem.
282, 13824-13832
| Abstract »
| Full Text »
| PDF »
- RNA interference based gene therapy for neurological disease.
- A. Jagannath and M. Wood (2007)
Brief Funct Genomic Proteomic
| Abstract »
| Full Text »
| PDF »
- Review of the Application of RNA Interference Technology in the Pharmaceutical Industry.
- H. M. Martineau and I. T. Pyrah (2007)
Toxicol Pathol
35, 327-336
| Abstract »
| Full Text »
| PDF »
- Comparison of siRNA-induced off-target RNA and protein effects.
- L. M. Aleman, J. Doench, and P. A. Sharp (2007)
RNA
13, 385-395
| Abstract »
| Full Text »
| PDF »
- Antiviral Protein APOBEC3G Localizes to Ribonucleoprotein Complexes Found in P Bodies and Stress Granules.
- S. Gallois-Montbrun, B. Kramer, C. M. Swanson, H. Byers, S. Lynham, M. Ward, and M. H. Malim (2007)
J. Virol.
81, 2165-2178
| Abstract »
| Full Text »
| PDF »
- QIP, a putative exonuclease, interacts with the Neurospora Argonaute protein and facilitates conversion of duplex siRNA into single strands.
- M. Maiti, H.-C. Lee, and Y. Liu (2007)
Genes & Dev.
21, 590-600
| Abstract »
| Full Text »
| PDF »
- Specific residues at every third position of siRNA shape its efficient RNAi activity.
- T. Katoh and T. Suzuki (2007)
Nucleic Acids Res.
35, e27
| Abstract »
| Full Text »
| PDF »
- Conversion of pre-RISC to holo-RISC by Ago2 during assembly of RNAi complexes.
- K. Kim, Y. S. Lee, and R. W. Carthew (2007)
RNA
13, 22-29
| Abstract »
| Full Text »
| PDF »
- Interactions between the RNA Interference Effector Protein Ago1 and 14-3-3 Proteins: CONSEQUENCES FOR CELL CYCLE PROGRESSION.
- C. Stoica, J. B. Carmichael, H. Parker, J. Pare, and T. C. Hobman (2006)
J. Biol. Chem.
281, 37646-37651
| Abstract »
| Full Text »
| PDF »
- The blossoming of RNA biology: Novel insights from plant systems.
- J. Bove, C. L.H. Hord, and M. A. Mullen (2006)
RNA
12, 2035-2046
| Full Text »
| PDF »
- Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense.
- X. Zhang, Y.-R. Yuan, Y. Pei, S.-S. Lin, T. Tuschl, D. J. Patel, and N.-H. Chua (2006)
Genes & Dev.
20, 3255-3268
| Abstract »
| Full Text »
| PDF »
- Effects of Dicer and Argonaute down-regulation on mRNA levels in human HEK293 cells.
- D. Schmitter, J. Filkowski, A. Sewer, R. S. Pillai, E. J. Oakeley, M. Zavolan, P. Svoboda, and W. Filipowicz (2006)
Nucleic Acids Res.
34, 4801-4815
| Abstract »
| Full Text »
| PDF »
- Competition for RISC binding predicts in vitro potency of siRNA.
- E. Koller, S. Propp, H. Murray, W. Lima, B. Bhat, T. P. Prakash, C. R. Allerson, E. E. Swayze, E. G. Marcusson, and N. M. Dean (2006)
Nucleic Acids Res.
34, 4467-4476
| Abstract »
| Full Text »
| PDF »
- Argonaute slicing is required for heterochromatic silencing and spreading..
- D. V. Irvine, M. Zaratiegui, N. H. Tolia, D. B. Goto, D. H. Chitwood, M. W. Vaughn, L. Joshua-Tor, and R. A. Martienssen (2006)
Science
313, 1134-1137
| Abstract »
| Full Text »
| PDF »
- A genomewide screen for components of the RNAi pathway in Drosophila cultured cells.
- S. Dorner, L. Lum, M. Kim, R. Paro, P. A. Beachy, and R. Green (2006)
PNAS
103, 11880-11885
| Abstract »
| Full Text »
| PDF »
- Small RNAs just got bigger: Piwi-interacting RNAs (piRNAs) in mammalian testes.
- V. N. Kim (2006)
Genes & Dev.
20, 1993-1997
| Abstract »
| Full Text »
| PDF »
- A brief history of RNAi: the silence of the genes.
- G. L. Sen and H. M. Blau (2006)
FASEB J
20, 1293-1299
| Abstract »
| Full Text »
| PDF »
- MicroRNA-Targeted and Small Interfering RNA-Mediated mRNA Degradation Is Regulated by Argonaute, Dicer, and RNA-Dependent RNA Polymerase in Arabidopsis.
- M. Ronemus, M. W. Vaughn, and R. A. Martienssen (2006)
PLANT CELL
18, 1559-1574
| Abstract »
| Full Text »
| PDF »
- Functional replacement of Trypanosoma brucei Argonaute by the human slicer Argonaute2.
- H. Shi, C. Tschudi, and E. Ullu (2006)
RNA
12, 943-947
| Abstract »
| Full Text »
| PDF »
- Genome-Wide Analysis of mRNAs Regulated by Drosha and Argonaute Proteins in Drosophila melanogaster.
- J. Rehwinkel, P. Natalin, A. Stark, J. Brennecke, S. M. Cohen, and E. Izaurralde (2006)
Mol. Cell. Biol.
26, 2965-2975
| Abstract »
| Full Text »
| PDF »
- Control of translation and mRNA degradation by miRNAs and siRNAs..
- M. A. Valencia-Sanchez, J. Liu, G. J. Hannon, and R. Parker (2006)
Genes & Dev.
20, 515-524
| Abstract »
| Full Text »
| PDF »
- Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA.
- H. Tsukioka, M. Takahashi, H. Mon, K. Okano, K. Mita, T. Shimada, J. M. Lee, Y. Kawaguchi, K. Koga, and T. Kusakabe (2006)
Nucleic Acids Res.
34, 1092-1101
| Abstract »
| Full Text »
| PDF »
- HelF, a putative RNA helicase acts as a nuclear suppressor of RNAi but not antisense mediated gene silencing.
- B. Popova, M. Kuhlmann, A. Hinas, F. Soderbom, and W. Nellen (2006)
Nucleic Acids Res.
34, 773-784
| Abstract »
| Full Text »
| PDF »
- Stem-cell protein Piwil2 is widely expressed in tumors and inhibits apoptosis through activation of Stat3/Bcl-XL pathway.
- J. H. Lee, D. Schutte, G. Wulf, L. Fuzesi, H.-J. Radzun, S. Schweyer, W. Engel, and K. Nayernia (2006)
Hum. Mol. Genet.
15, 201-211
| Abstract »
| Full Text »
| PDF »
- The RNAi Pathway Initiated by Dicer-2 in Drosophila.
- K. KIM, Y.S. LEE, D. HARRIS, K. NAKAHARA, and R.W. CARTHEW (2006)
Cold Spring Harb Symp Quant Biol
71, 39-44
| Abstract »
| PDF »
- Molecular Mechanism of Target RNA Transcript Recognition by Argonaute-Guide Complexes.
- J.S. PARKER, S.M. ROE, and D. BARFORD (2006)
Cold Spring Harb Symp Quant Biol
71, 45-50
| Abstract »
| PDF »
- The Argonautes.
- L. JOSHUA-TOR (2006)
Cold Spring Harb Symp Quant Biol
71, 67-72
| Abstract »
| PDF »
- Structure of Dicer and Mechanistic Implications for RNAi.
- I.J. MacRAE, F. LI, K. ZHOU, W.Z. CANDE, and J.A. DOUDNA (2006)
Cold Spring Harb Symp Quant Biol
71, 73-80
| Abstract »
| PDF »
- Structural Biology of RNA Silencing and Its Functional Implications.
- D.J. PATEL, J.-B. MA, Y.-R. YUAN, K. YE, Y. PEI, V. KURYAVYI, L. MALININA, G. MEISTER, and T. TUSCHL (2006)
Cold Spring Harb Symp Quant Biol
71, 81-93
| Abstract »
| PDF »
- The Caenorhabditis elegans Argonautes ALG-1 and ALG-2: Almost Identical yet Different.
- B.B.J. TOPS, R.H.A. PLASTERK, and R.F. KETTING (2006)
Cold Spring Harb Symp Quant Biol
71, 189-194
| Abstract »
| PDF »
- Relationship between Retroviral Replication and RNA Interference Machineries.
- K. MOELLING, A. MATSKEVICH, and J.-S. JUNG (2006)
Cold Spring Harb Symp Quant Biol
71, 365-368
| Abstract »
| PDF »
- RNAi-mediated Heterochromatin Assembly in Fission Yeast.
- M. ZOFALL and S.I.S. GREWAL (2006)
Cold Spring Harb Symp Quant Biol
71, 487-496
| Abstract »
| PDF »
- Slicing and Spreading of Heterochromatic Silencing by RNA Interference.
- S.M. LOCKE and R.A. MARTIENSSEN (2006)
Cold Spring Harb Symp Quant Biol
71, 497-503
| Abstract »
| PDF »
- The Expanding Universe of Noncoding RNAs.
- G.J. HANNON, F.V. RIVAS, E.P. MURCHISON, and J.A. STEITZ (2006)
Cold Spring Harb Symp Quant Biol
71, 551-564
| Abstract »
| PDF »
- Small interfering RNAs containing full 2'-O-methylribonucleotide-modified sense strands display Argonaute2/eIF2C2-dependent activity.
- B. A. KRAYNACK and B. F. BAKER (2006)
RNA
12, 163-176
| Abstract »
| Full Text »
| PDF »
- A human, ATP-independent, RISC assembly machine fueled by pre-miRNA.
- E. Maniataki and Z. Mourelatos (2005)
Genes & Dev.
19, 2979-2990
| Abstract »
| Full Text »
| PDF »
- MicroRNA function: Multiple mechanisms for a tiny RNA?.
- R. S. PILLAI (2005)
RNA
11, 1753-1761
| Abstract »
| Full Text »
| PDF »
- The Putative NTPase Fap7 Mediates Cytoplasmic 20S Pre-rRNA Processing through a Direct Interaction with Rps14.
- S. Granneman, M. R. Nandineni, and S. J. Baserga (2005)
Mol. Cell. Biol.
25, 10352-10364
| Abstract »
| Full Text »
| PDF »
- Molecular Requirements for RNA-induced Silencing Complex Assembly in the Drosophila RNA Interference Pathway.
- J. W. Pham and E. J. Sontheimer (2005)
J. Biol. Chem.
280, 39278-39283
| Abstract »
| Full Text »
| PDF »
- Targeting MLL-AF4 with short interfering RNAs inhibits clonogenicity and engraftment of t(4;11)-positive human leukemic cells.
- M. Thomas, A. Gessner, H.-P. Vornlocher, P. Hadwiger, J. Greil, and O. Heidenreich (2005)
Blood
106, 3559-3566
| Abstract »
| Full Text »
| PDF »
- Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs.
- C. K. RAYMOND, B. S. ROBERTS, P. GARRETT-ENGELE, L. P. LIM, and J. M. JOHNSON (2005)
RNA
11, 1737-1744
| Abstract »
| Full Text »
| PDF »
- ATP modulates siRNA interactions with an endogenous human Dicer complex.
- J. L. PELLINO, L. JASKIEWICZ, W. FILIPOWICZ, and E. J. SONTHEIMER (2005)
RNA
11, 1719-1724
| Abstract »
| Full Text »
| PDF »
- microPrimer: the biogenesis and function of microRNA.
- T. Du and P. D. Zamore (2005)
Development
132, 4645-4652
| Abstract »
| Full Text »
| PDF »
- Ribo-gnome: The Big World of Small RNAs.
- P. D. Zamore and B. Haley (2005)
Science
309, 1519-1524
| Abstract »
| Full Text »
| PDF »
- Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs.
- N. Baumberger and D. C. Baulcombe (2005)
PNAS
102, 11928-11933
| Abstract »
| Full Text »
| PDF »
- Characterization of a highly variable eutherian microRNA gene.
- H. B. HOUBAVIY, L. DENNIS, R. JAENISCH, and P. A. SHARP (2005)
RNA
11, 1245-1257
| Abstract »
| Full Text »
| PDF »
- RNA meets chromatin.
- E. Bernstein and C. D. Allis (2005)
Genes & Dev.
19, 1635-1655
| Abstract »
| Full Text »
| PDF »
|
|