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Science 20 August 2004:
Vol. 305. no. 5687, pp. 1150 - 1153
DOI: 10.1126/science.1099159
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Reports

Requirement of Rac1 and Rac2 Expression by Mature Dendritic Cells for T Cell Priming

Federica Benvenuti,1*{dagger} Stephanie Hugues,1{dagger} Marita Walmsley,2 Sandra Ruf,2 Luc Fetler,3 Michel Popoff,4 Victor L. J. Tybulewicz,2 Sebastian Amigorena1{ddagger}

Upon maturation, dendritic cells (DCs) acquire the unique ability to activate naïve T cells. We used time-lapse video microscopy and two-photon imaging of intact lymph nodes to showthat after establishing initial contact between their dendrites and naïve T lymphocytes, mature DCs migrate toward the contacted lymphocytes. Subsequently, the DCs tightly entrap the T cells within a complex net of membrane extensions. The Rho family guanosine triphosphatases Rac1 and Rac2 but not Rho itself control the formation of dendrites in mature DCs, their polarized short-range migration toward T cells, and T cell priming.

1 Unité INSERM 365, Institut Curie, 26 rue d'Ulm, 75005 Paris, France.
2 Division of Immune Cell Biology, National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK.
3 UMR 168 CNRS/Institut Curie, 11 rue Pierre et Marie Curie, 75005 Paris, France.
4 Institut Pasteur, Unité des Toxines Microbiennes, 75015 Paris, France.



* Present address: International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34121 Trieste, Italy.

{dagger} These authors contributed equally to this work.

{ddagger} To whom correspondence should be addressed. E-mail: sebastian.amigorena{at}curie.fr

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