Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 13 August 2004:
Vol. 305. no. 5686, pp. 1007 - 1009
DOI: 10.1126/science.1100035
Prev | Table of Contents | Next

Reports

Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy

Jan Huisken,* Jim Swoger, Filippo Del Bene, Joachim Wittbrodt, Ernst H. K. Stelzer*

Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination microscopy (SPIM) to generate multidimensional images of samples up to a few millimeters in size. The system combines two-dimensional illumination with orthogonal camera-based detection to achieve high-resolution, optically sectioned imaging throughout the sample, with minimal photodamage and at speeds capable of capturing transient biological phenomena. We used SPIM to visualize all muscles in vivo in the transgenic Medaka line Arnie, which expresses green fluorescent protein in muscle tissue. We also demonstrate that SPIM can be applied to visualize the embryogenesis of the relatively opaque Drosophila melanogaster in vivo.

European Molecular Biology Laboratory (EMBL), Meyerhofstraße 1, D-69117 Heidelberg, Germany.

* To whom correspondence should be addressed. E-mail: huisken{at}embl.de (J.H.) and stelzer{at}embl.de (E.H.K.S.)

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Optical control of zebrafish behavior with halorhodopsin.
A. B. Arrenberg, F. Del Bene, and H. Baier (2009)
PNAS 106, 17968-17973
   Abstract »    Full Text »    PDF »
Arterial-Venous Segregation by Selective Cell Sprouting: An Alternative Mode of Blood Vessel Formation.
S. P. Herbert, J. Huisken, T. N. Kim, M. E. Feldman, B. T. Houseman, R. A. Wang, K. M. Shokat, and D. Y. R. Stainier (2009)
Science 326, 294-298
   Abstract »    Full Text »    PDF »
ojoplano-mediated basal constriction is essential for optic cup morphogenesis.
J. R. Martinez-Morales, M. Rembold, K. Greger, J. C. Simpson, K. E. Brown, R. Quiring, R. Pepperkok, M. D. Martin-Bermudo, H. Himmelbauer, and J. Wittbrodt (2009)
Development 136, 2165-2175
   Abstract »    Full Text »    PDF »
Selective plane illumination microscopy techniques in developmental biology.
J. Huisken and D. Y. R. Stainier (2009)
Development 136, 1963-1975
   Abstract »    Full Text »    PDF »
Genetic Evidence for a Noncanonical Function of Seryl-tRNA Synthetase in Vascular Development.
W. Herzog, K. Muller, J. Huisken, and D. Y.R. Stainier (2009)
Circ. Res. 104, 1260-1266
   Abstract »    Full Text »    PDF »
Multilayer three-dimensional super resolution imaging of thick biological samples.
A. Vaziri, J. Tang, H. Shroff, and C. V. Shank (2008)
PNAS 105, 20221-20226
   Abstract »    Full Text »    PDF »
Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy.
P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H.K. Stelzer (2008)
Science 322, 1065-1069
   Abstract »    Full Text »    PDF »
In Vivo Imaging of the Inflammatory Receptor CD40 After Cerebral Ischemia Using a Fluorescent Antibody.
J. Klohs, M. Grafe, K. Graf, J. Steinbrink, T. Dietrich, D. Stibenz, P. Bahmani, G. Kronenberg, C. Harms, M. Endres, et al. (2008)
Stroke 39, 2845-2852
   Abstract »    Full Text »    PDF »
High-speed imaging of developing heart valves reveals interplay of morphogenesis and function.
P. J. Scherz, J. Huisken, P. Sahai-Hernandez, and D. Y. R. Stainier (2008)
Development 135, 1179-1187
   Abstract »    Full Text »    PDF »
Zebrafish model for human long QT syndrome.
R. Arnaout, T. Ferrer, J. Huisken, K. Spitzer, D. Y. R. Stainier, M. Tristani-Firouzi, and N. C. Chi (2007)
PNAS 104, 11316-11321
   Abstract »    Full Text »    PDF »
Visualizing Plant Development and Gene Expression in Three Dimensions Using Optical Projection Tomography.
K. Lee, J. Avondo, H. Morrison, L. Blot, M. Stark, J. Sharpe, A. Bangham, and E. Coen (2006)
PLANT CELL 18, 2145-2156
   Abstract »    Full Text »    PDF »
From imaging to understanding: Frontiers in Live Cell Imaging, Bethesda, MD, April 19-21, 2006.
Y.-l. Wang, K. M. Hahn, R. F. Murphy, and A. F. Horwitz (2006)
J. Cell Biol. 174, 481-484
   Abstract »    Full Text »    PDF »
Forebrain Gonadotropin-Releasing Hormone Neuronal Development: Insights from Transgenic Medaka and the Relevance to X-Linked Kallmann Syndrome.
K. Okubo, F. Sakai, E. L. Lau, G. Yoshizaki, Y. Takeuchi, K. Naruse, K. Aida, and Y. Nagahama (2006)
Endocrinology 147, 1076-1084
   Abstract »    Full Text »    PDF »
MEPD: a resource for medaka gene expression patterns.
T. Henrich, M. Ramialison, B. Wittbrodt, B. Assouline, F. Bourrat, A. Berger, H. Himmelbauer, T. Sasaki, N. Shimizu, M. Westerfield, et al. (2005)
Bioinformatics 21, 3195-3197
   Abstract »    Full Text »    PDF »
Two-Photon Microscopy of Cells and Tissue.
M. Rubart (2004)
Circ. Res. 95, 1154-1166
   Abstract »    Full Text »    PDF »
How to assess toxin ingestion and post-ingestion partitioning in zooplankton?.
G. S. Caldwell, S. B. Watson, and M. G. Bentley (2004)
J. Plankton Res. 26, 1369-1377
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)