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Science 23 July 2004:
Vol. 305. no. 5683, pp. 522 - 525
DOI: 10.1126/science.1095209
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Reports

A Synthetic Conjugate Polysaccharide Vaccine Against Haemophilus influenzae Type b

V. Verez-Bencomo,1* V. Fernández-Santana,1* Eugenio Hardy,2* Maria E. Toledo,3* Maria C. Rodríguez,1 Lazaro Heynngnezz,2 Arlene Rodriguez,2 Alberto Baly,3 Luis Herrera,2 Mabel Izquierdo,2 Annette Villar,1 Yury Valdés,1 Karelia Cosme,2 Mercedes L. Deler,1 Manuel Montane,2 Ernesto Garcia,1 Alexis Ramos,1 Aristides Aguilar,2 Ernesto Medina,2 Gilda Toraño,3 Iván Sosa,2 Ibis Hernandez,3 Raydel Martínez,3 Alexis Muzachio,2 Ania Carmenates,4 Lourdes Costa,2 Félix Cardoso,1 Concepción Campa,5 Manuel Diaz,3 René Roy6*

Glycoconjugate vaccines provide effective prophylaxis against bacterial infections. To date, however, no commercial vaccine has been available in which the key carbohydrate antigens are produced synthetically. We describe the large-scale synthesis, pharmaceutical development, and clinical evaluation of a conjugate vaccine composed of a synthetic capsular polysaccharide antigen of Haemophilus influenzae type b (Hib). The vaccine was evaluated in clinical trials in Cuba and showed long-term protective antibody titers that compared favorably to licensed products prepared with the Hib polysaccharide extracted from bacteria. This demonstrates that access to synthetic complex carbohydrate–based vaccines is feasible and provides a basis for further development of similar approaches for other human pathogens.

1 Center for the Study of Synthetic Antigens, Facultad de Química, Universidad de la Habana, Ciudad Habana, Cuba, 10400.
2 Center for Genetic Engineering and Biotechnology, Apdo 6162, Cubanacan, Playa, Ciudad Habana, Cuba 10600.
3 Institute for Tropical Medicine Pedro Kouri, Autopista Novia del Mediodía, Km 6, La Lisa, Apdo 601, Marianao 13, Ciudad Habana, Cuba 11300.
4 Provincial Center for Hygiene and Epidemiology, Camaguey, Cuba.
5 Finlay Institute, Havana, Cuba 11600.
6 Department of Chemistry, Université du Québec à Montréal, Post Office Box 8888, Succursale Centre-Ville, Montreal, Québec H3C 3P8, Canada.

* To whom correspondence should be addressed. E-mail: vicente{at}fq.uh.cu (V.V.-B.); violeta{at}fq.uh.cu (V.F.-S.); eugenio.hardy{at}cigb.edu.cu (E.H.); mariaeugenia{at}ipk.sld.cu (M.E.T.); roy.rene{at}uqam.ca (R.R.)

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Science. ISSN 0036-8075 (print), 1095-9203 (online)