Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Nanomedicine Summit 2008

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 16 July 2004:
Vol. 305. no. 5682, pp. 393 - 396
DOI: 10.1126/science.1099892
Prev | Table of Contents | Next

Reports

Spatial Coordination of Cytokinetic Events by Compartmentalization of the Cell Cortex

Jeroen Dobbelaere and Yves Barral*

During cytokinesis, furrow ingression and plasma membrane fission irreversibly separate daughter cells. How actomyosin ring assembly and contraction, vesicle fusion, and abscission are spatially coordinated was unknown. We found that during cytokinesis septin rings, located on both sides of the actomyosin ring, acted as barriers to compartmentalize the cortex around the cleavage site. Compartmentalization maintained diffusible cortical factors, such as the exocyst and the polarizome, to the site of cleavage. In turn, such factors were required for actomyosin ring function and membrane abscission. Thus, a specialized cortical compartment ensures the spatial coordination of cytokinetic events.

Institute of Biochemistry, Swiss Federal Institute of Technology, ETH-Hönggerberg, 8093 Zürich, Switzerland.

* To whom correspondence should be addressed. E-mail: yves.barral{at}bc.biol.ethz.ch

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Role of Nucleotide Binding in Septin-Septin Interactions and Septin Localization in Saccharomyces cerevisiae.
S. Nagaraj, A. Rajendran, C. E. Jackson, and M. S. Longtine (2008)
Mol. Cell. Biol. 28, 5120-5137
   Abstract »    Full Text »    PDF »
Saccharomyces cerevisiae Afr1 Protein Is a Protein Phosphatase 1/Glc7-Targeting Subunit That Regulates the Septin Cytoskeleton during Mating.
J. P. Bharucha, J. R. Larson, J. B. Konopka, and K. Tatchell (2008)
Eukaryot. Cell 7, 1246-1255
   Abstract »    Full Text »    PDF »
Septins Stabilize Mitochondria in Tetrahymena thermophila.
D. Wloga, I. Strzyzewska-Jowko, J. Gaertig, and M. Jerka-Dziadosz (2008)
Eukaryot. Cell 7, 1373-1386
   Abstract »    Full Text »    PDF »
Saccharomyces cerevisiae septins: Supramolecular organization of heterooligomers and the mechanism of filament assembly.
A. Bertin, M. A. McMurray, P. Grob, S.-S. Park, G. Garcia III, I. Patanwala, H.-l. Ng, T. Alber, J. Thorner, and E. Nogales (2008)
PNAS 105, 8274-8279
   Abstract »    Full Text »    PDF »
Zygotically controlled F-actin establishes cortical compartments to stabilize furrows during Drosophila cellularization.
A. M. Sokac and E. Wieschaus (2008)
J. Cell Sci. 121, 1815-1824
   Abstract »    Full Text »    PDF »
Cdc42 and the Ste20-like kinase Don3 act independently in triggering cytokinesis in Ustilago maydis.
C. Bohmer, M. Bohmer, M. Bolker, and B. Sandrock (2008)
J. Cell Sci. 121, 143-148
   Abstract »    Full Text »    PDF »
Identification of Yeast IQGAP (Iqg1p) as an Anaphase-Promoting-Complex Substrate and Its Role in Actomyosin-Ring-Independent Cytokinesis.
N. Ko, R. Nishihama, G. H. Tully, D. Ostapenko, M. J. Solomon, D. O. Morgan, and J. R. Pringle (2007)
Mol. Biol. Cell 18, 5139-5153
   Abstract »    Full Text »    PDF »
Sequential and Distinct Roles of the Cadherin Domain-containing Protein Axl2p in Cell Polarization in Yeast Cell Cycle.
X.-D. Gao, L. M. Sperber, S. A. Kane, Z. Tong, A. H. Y. Tong, C. Boone, and E. Bi (2007)
Mol. Biol. Cell 18, 2542-2560
   Abstract »    Full Text »    PDF »
Candida albicans hyphal morphogenesis occurs in Sec3p-independent and Sec3p-dependent phases separated by septin ring formation.
C.-R. Li, R. T.-H. Lee, Y.-M. Wang, X.-D. Zheng, and Y. Wang (2007)
J. Cell Sci. 120, 1898-1907
   Abstract »    Full Text »    PDF »
Sterol-Rich Plasma Membrane Domains in Fungi.
F. J. Alvarez, L. M. Douglas, and J. B. Konopka (2007)
Eukaryot. Cell 6, 755-763
   Full Text »    PDF »
Yeast Formins Bni1 and Bnr1 Utilize Different Modes of Cortical Interaction during the Assembly of Actin Cables.
S. M. Buttery, S. Yoshida, and D. Pellman (2007)
Mol. Biol. Cell 18, 1826-1838
   Abstract »    Full Text »    PDF »
Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond.
H.-O. Park and E. Bi (2007)
Microbiol. Mol. Biol. Rev. 71, 48-96
   Abstract »    Full Text »    PDF »
RalA-exocyst-dependent Recycling Endosome Trafficking Is Required for the Completion of Cytokinesis.
X.-W. Chen, M. Inoue, S. C. Hsu, and A. R. Saltiel (2006)
J. Biol. Chem. 281, 38609-38616
   Abstract »    Full Text »    PDF »
The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism.
J. B. Moseley and B. L. Goode (2006)
Microbiol. Mol. Biol. Rev. 70, 605-645
   Abstract »    Full Text »    PDF »
Cell Wall Assembly in Saccharomyces cerevisiae.
G. Lesage and H. Bussey (2006)
Microbiol. Mol. Biol. Rev. 70, 317-343
   Abstract »    Full Text »    PDF »
The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis.
M. K. Gatt and D. M. Glover (2006)
J. Cell Sci. 119, 2225-2235
   Abstract »    Full Text »    PDF »
Role of a Cdc42p Effector Pathway in Recruitment of the Yeast Septins to the Presumptive Bud Site.
M. Iwase, J. Luo, S. Nagaraj, M. Longtine, H. B. Kim, B. K. Haarer, C. Caruso, Z. Tong, J. R. Pringle, and E. Bi (2006)
Mol. Biol. Cell 17, 1110-1125
   Abstract »    Full Text »    PDF »
Here come the septins: novel polymers that coordinate intracellular functions and organization.
E. T. Spiliotis and W. J. Nelson (2006)
J. Cell Sci. 119, 4-10
   Abstract »    Full Text »    PDF »
A novel role for the CBF3 kinetochore-scaffold complex in regulating septin dynamics and cytokinesis.
A. N. Gillis, S. Thomas, S. D. Hansen, and K. B. Kaplan (2005)
J. Cell Biol. 171, 773-784
   Abstract »    Full Text »    PDF »
Role of Septins and the Exocyst Complex in the Function of Hydrolytic Enzymes Responsible for Fission Yeast Cell Separation.
A. B. Martin-Cuadrado, J. L. Morrell, M. Konomi, H. An, C. Petit, M. Osumi, M. Balasubramanian, K. L. Gould, F. del Rey, and C. R. V. de Aldana (2005)
Mol. Biol. Cell 16, 4867-4881
   Abstract »    Full Text »    PDF »
Septin Function in Yeast Model Systems and Pathogenic Fungi.
L. M. Douglas, F. J. Alvarez, C. McCreary, and J. B. Konopka (2005)
Eukaryot. Cell 4, 1503-1512
   Full Text »    PDF »
Role of the Septin Ring in the Asymmetric Localization of Proteins at the Mother-Bud Neck in Saccharomyces cerevisiae.
L. Kozubowski, J. R. Larson, and K. Tatchell (2005)
Mol. Biol. Cell 16, 3455-3466
   Abstract »    Full Text »    PDF »
Septin-dependent compartmentalization of the endoplasmic reticulum during yeast polarized growth.
C. Luedeke, S. B. Frei, I. Sbalzarini, H. Schwarz, A. Spang, and Y. Barral (2005)
J. Cell Biol. 169, 897-908
   Abstract »    Full Text »    PDF »
Cell Cycle-regulated Trafficking of Chs2 Controls Actomyosin Ring Stability during Cytokinesis.
L. VerPlank and R. Li (2005)
Mol. Biol. Cell 16, 2529-2543
   Abstract »    Full Text »    PDF »
The kinetochore protein Ndc10p is required for spindle stability and cytokinesis in yeast.
D. C. Bouck and K. S. Bloom (2005)
PNAS 102, 5408-5413
   Abstract »    Full Text »    PDF »
A Mitotic Septin Scaffold Required for Mammalian Chromosome Congression and Segregation.
E. T. Spiliotis, M. Kinoshita, and W. J. Nelson (2005)
Science 307, 1781-1785
   Abstract »    Full Text »    PDF »
Actin and Septin Ultrastructures at the Budding Yeast Cell Cortex.
A. A. Rodal, L. Kozubowski, B. L. Goode, D. G. Drubin, and J. H. Hartwig (2005)
Mol. Biol. Cell 16, 372-384
   Abstract »    Full Text »    PDF »
Corralled by septin.
W. A. Wells (2004)
J. Cell Biol. 166, 445
   Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)