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Science 2 July 2004:
Vol. 305. no. 5680, pp. 90 - 93
DOI: 10.1126/science.1098925
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Reports

Lack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia

Robert G. Harris,* Erica L. Herzog,* Emanuela M. Bruscia, Joanna E. Grove, John S. Van Arnam, Diane S. Krause{dagger}

Analysis of developmental plasticity of bone marrow–derived cells (BMDCs) is complicated by the possibility of cell-cell fusion. Here we demonstrate that epithelial cells can develop from BMDCs without cell-cell fusion. We use the Cre/lox system together with ß-galactosidase and enhanced green fluorescent protein expression in transgenic mice to identify epithelial cells in the lung, liver, and skin that develop from BMDCs without cell fusion.

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.


* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: diane.krause{at}yale.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)