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Originally published in Science Express on 27 May 2004
Science 2 July 2004:
Vol. 305. no. 5680, pp. 61 - 66
DOI: 10.1126/science.1097931
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Research Articles

Dissection of the Mammalian Midbody Proteome Reveals Conserved Cytokinesis Mechanisms

Ahna R. Skop,1,2*{dagger} Hongbin Liu,3 John Yates, III,3 Barbara J. Meyer,1,2 Rebecca Heald1

Cytokinesis is the essential process that partitions cellular contents into daughter cells. To identify and characterize cytokinesis proteins rapidly, we used a functional proteomic and comparative genomic strategy. Midbodies were isolated from mammalian cells, proteins were identified by multidimensional protein identification technology (MudPIT), and protein function was assessed in Caenorhabditis elegans. Of 172 homologs disrupted by RNA interference, 58% displayed defects in cleavage furrow formation or completion, or germline cytokinesis. Functional dissection of the midbody demonstrated the importance of lipid rafts and vesicle trafficking pathways in cytokinesis, and the utilization of common membrane cytoskeletal components in diverse morphogenetic events in the cleavage furrow, the germline, and neurons.

1 Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA.
2 Howard Hughes Medical Institute, Berkeley, CA 94720, USA.
3 The Scripps Research Institute, Department of Cell Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.



{dagger} Present address: Department of Genetics, University of Wisconsin–Madison, Madison, WI 53706, USA.

* To whom correspondence should be addressed: E-mail: skop{at}wisc.edu

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