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Science 18 June 2004:
Vol. 304. no. 5678, pp. 1797 - 1800
DOI: 10.1126/science.1099754
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Reports

Dynamics of Single mRNPs in Nuclei of Living Cells

Yaron Shav-Tal,1 Xavier Darzacq,1 Shailesh M. Shenoy,1 Dahlene Fusco,1 Susan M. Janicki,2 David L. Spector,2 Robert H. Singer1*

Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA)expression to characterize the movement in real time of single mRNA-protein complexes (mRNPs) in the nucleus of living mammalian cells. This mobility was not directed but was governed by simple diffusion. Some mRNPs were partially corralled throughout the nonhomogenous nuclear environment, but no accumulation at subnuclear domains was observed. Following energy deprivation, energy-independent motion of mRNPs was observed in a highly ATP-dependent nuclear environment; movements were constrained to chromatin-poor domains and excluded by newly formed chromatin barriers. This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model.

1 Departments of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
2 Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.

* To whom correspondence should be addressed. E-mail: rhsinger{at}aecom.yu.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)