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Science 4 June 2004:
Vol. 304. no. 5676, pp. 1513 - 1515
DOI: 10.1126/science.1097404
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Reports

A Microdomain for Protein Secretion in Gram-Positive Bacteria

Jason Rosch and Michael Caparon*

Gram-positive bacteria face unique challenges in generating biologically active conformations for their exported proteins because they lack a dedicated compartment for folding secreted polypeptides. We have discovered that protein secretion by way of the general secretory (Sec) pathway in the important human pathogen Streptococcus pyogenes proceeds through a single microdomain. Unlike other mechanisms for asymmetry involving the Sec pathway, proteins destined for secretion are targeted to a single locus distal to either cell pole that has specialized to contain the Sec translocons. This subcellular organization may represent a paradigm for secretion common to other Gram-positive pathogens with profound implications for pathogenesis.

Department of Molecular Microbiology, Washington University School of Medicine, Box 8230, St. Louis, MO 63110–1093, USA.

* To whom correspondence should be addressed. E-mail: caparon{at}borcim.wustl.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)