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Definition of a Bacterial Type IV Secretion Pathway for a DNA Substrate
Eric Cascales and
Peter J. Christie*
Bacteria use conjugation systems, a subfamily of the type IVsecretion systems, to transfer DNA to recipient cells. Despite50 years of research, the architecture and mechanism of actionof the channel mediating DNA transfer across the bacterial cellenvelope remains obscure. By use of a sensitive, quantifiableassay termed transfer DNA immunoprecipitation (TrIP), we identifycontacts between a DNA substrate (T-DNA) and 6 of 12 componentsof the VirB/D4 conjugation system of the phytopathogen Agrobacteriumtumefaciens. Our results define the translocation pathway fora DNA substrate through a bacterial conjugation machine, specifyingthe contributions of each subunit of the secretory apparatusto substrate passage.
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, JFB1.765, Houston, TX 77030, USA.
* To whom correspondence should be addressed. E-mail: peter.j.christie{at}uth.tmc.edu
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