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Science 14 May 2004:
Vol. 304. no. 5673, pp. 987 - 990
DOI: 10.1126/science.1094567
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Reports

Continuous Particle Separation Through Deterministic Lateral Displacement

Lotien Richard Huang,1 Edward C. Cox,2 Robert H. Austin,3 James C. Sturm1

We report on a microfluidic particle-separation device that makes use of the asymmetric bifurcation of laminar flow around obstacles. A particle chooses its path deterministically on the basis of its size. All particles of a given size follow equivalent migration paths, leading to high resolution. The microspheres of 0.8, 0.9, and 1.0 micrometers that were used to characterize the device were sorted in 40seconds with a resolution of ~10nanometers, which was better than the time and resolution of conventional flow techniques. Bacterial artificial chromosomes could be separated in 10 minutes with a resolution of ~12%.

1 Department of Electrical Engineering, Princeton Institute for the Science and Technology of Materials (PRISM), Princeton University, Princeton, NJ 08544, USA.
2 Department of Molecular Biology, Princeton Institute for the Science and Technology of Materials (PRISM), Princeton University, Princeton, NJ 08544, USA.
3 Department of Physics, Princeton Institute for the Science and Technology of Materials (PRISM), Princeton University, Princeton, NJ 08544, USA.

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