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Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility
Mousumi Ghosh,1Xiaoyan Song,1Ghassan Mouneimne,1Mazen Sidani,1David S. Lawrence,2John S. Condeelis1*
A general caging method for proteins that are regulated by phosphorylationwas used to study the in vivo biochemical action of cofilinand the subsequent cellular response. By acute and local activationof a chemically engineered, light-sensitive phosphocofilin mimic,we demonstrate that cofilin polymerizes actin, generates protrusions,and determines the direction of cell migration. We propose arole for cofilin that is distinct from its role as an actin-depolymerizingfactor.
1 Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA. 2 Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
* To whom correspondence should be addressed. E-mail: condeeli{at}aecom.yu.edu
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