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Science 9 April 2004:
Vol. 304. no. 5668, pp. 286 - 289
DOI: 10.1126/science.1095049
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Reports

Tip20p Prohibits Back-Fusion of COPII Vesicles with the Endoplasmic Reticulum

Faustin Kamena and Anne Spang*

Directionality in intracellular trafficking is essential to ensure the correct localization of proteins along the secretory pathway. Here, we found evidence for an active mechanism that prohibited back-fusion of de novo–generated vesicles with their donor compartment. Tip20p is a peripheral membrane protein implicated in consumption of COPI vesicles at the endoplasmic reticulum. However, a specific mutant of TIP20 did not interfere with COPII vesicle generation but allowed these vesicles to fuse back to the endoplasmic reticulum, a process that does not occur normally in the cell.

Friedrich Miescher Laboratorium der Max Planck Gesellschaft, Spemannstrasse 39, D-72076 Tübingen, Germany.

* To whom correspondence should be addressed. E-mail: anne.spang{at}tuebingen.mpg.de

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