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Originally published in Science Express on 12 February 2004
Science 12 March 2004:
Vol. 303. no. 5664, pp. 1669 - 1674
DOI: 10.1126/science.1094515
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Reports

Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst

Woo Suk Hwang,1,2* Young June Ryu,1 Jong Hyuk Park,3 Eul Soon Park,1 Eu Gene Lee,1 Ja Min Koo,4 Hyun Yong Jeon,1 Byeong Chun Lee,1 Sung Keun Kang,1 Sun Jong Kim,3 Curie Ahn,5 Jung Hye Hwang,6 Ky Young Park,7 Jose B. Cibelli,8 Shin Yong Moon5*

Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.

1 College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
2 School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
3 Medical Research Center, MizMedi Hospital, Seoul, 135-280, Korea.
4 Gachon Medical School, Incheon, 417-840, Korea.
5 College of Medicine, Seoul National University, Seoul, 110-744, Korea.
6 School of Medicine, Hanyang University, Seoul, 471-701, Korea.
7 College of Natural Science, Sunchon National University, Sunchon, 540-742, Korea.
8 Department of Animal Science-Physiology, Michigan State University, East Lansing, MI 48824, USA.

* To whom correspondence should be addressed. E-mail: hwangws{at}snu.ac.kr (W.S.H.); shmoon{at}plaza.snu.ac.kr (S.Y.M.)

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