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Science 5 March 2004:
Vol. 303. no. 5663, pp. 1516 - 1519
DOI: 10.1126/science.1093586
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Reports

A Membrane-Anchored Protein Kinase Involved in Brassica Self-Incompatibility Signaling

Kohji Murase,1 Hiroshi Shiba,1 Megumi Iwano,1 Fang-Sik Che,1 Masao Watanabe,2 Akira Isogai,1 Seiji Takayama1*

Self-incompatibility (SI) response in Brassica is initiated by haplotype-specific interactions between the pollen-borne ligand S locus protein 11/SCR and its stigmatic S receptor kinase, SRK. This binding induces autophosphorylation of SRK, which is then thought to trigger a signaling cascade that leads to self-pollen rejection. A recessive mutation of the modifier (m) gene eliminates the SI response in stigma. Positional cloning of M has revealed that it encodes a membrane-anchored cytoplasmic serine/threonine protein kinase, designated M locus protein kinase (MLPK). Transient expression of MLPK restores the ability of mm papilla cells to reject self-pollen, suggesting that MLPK is a positive mediator of Brassica SI signaling.

1 Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.
2 Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.

* To whom correspondence should be addressed. E-mail: takayama{at}bs.aist-nara.ac.jp

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