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ReportsMinimal Machinery of RNA Polymerase Holoenzyme Sufficient for Promoter Melting![]()
We determined the minimal portion of Escherichia coli RNA polymerase (RNAP) holoenzyme able to accomplish promoter melting, the crucial step in transcription initiation that provides RNAP access to the template strand. Upon duplex DNA binding, the N terminus of the ß' subunit (amino acids 1 to 314) and amino acids 94 to 507 of the Departments of Stomatology and Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.
* These authors contributed equally to the work.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)