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Science 16 January 2004:
Vol. 303. no. 5656, pp. 363 - 366
DOI: 10.1126/science.1090908
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Reports

Guar Seed ß-Mannan Synthase Is a Member of the Cellulose Synthase Super Gene Family

Kanwarpal S. Dhugga,1* Roberto Barreiro,1 Brad Whitten,1 Kevin Stecca,3 Jan Hazebroek,1 Gursharn S. Randhawa,2 Maureen Dolan,3 Anthony J. Kinney,3 Dwight Tomes,1 Scott Nichols,4 Paul Anderson1

Genes for the enzymes that make plant cell wall hemicellulosic polysaccharides remain to be identified. We report here the isolation of a complementary DNA (cDNA) clone encodingone such enzyme, mannan synthase (ManS), that makes the ß-1, 4-mannan backbone of galactomannan, a hemicellulosic storage polysaccharide in guar seed endosperm walls. The soybean somatic embryos expressing ManS cDNA contained high levels of ManS activities that localized to Golgi. Phylogenetically, ManS is closest to group A of the cellulose synthase–like (Csl) sequences from Arabidopsis and rice. Our results provide the biochemical proof for the involvement of the Csl genes in ß-glycan formation in plants.

1 Crop Genetics Research and Development, Pioneer Hi-Bred International, Inc., A DuPont Company, 7300 NW 62nd Avenue, Johnston, IA 50131, USA.
2 Department of Biosciences and Biotechnology, Indian Institute of Technology, Roorkee, India 247667.
3 Crop Genetics Research and Development, DuPont Experiment Station, Wilmington, DE 19880, USA.
4 Central Research and Development, DuPont Experiment Station, Wilmington, DE 19880, USA.

* To whom correspondence should be addressed. E-mail: Kanwarpal.Dhugga{at}Pioneer.Com

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