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Science 19 December 2003:
Vol. 302. no. 5653, pp. 2126 - 2130
DOI: 10.1126/science.1085432
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Reports

Dual Activation of the Drosophila Toll Pathway by Two Pattern Recognition Receptors

Vanessa Gobert,1 Marie Gottar,1 Alexey A. Matskevich,1 Sophie Rutschmann,1* Julien Royet,1 Marcia Belvin,2 Jules A. Hoffmann,1 Dominique Ferrandon1{dagger}

The Toll-dependent defense against Gram-positive bacterial infections in Drosophila is mediated through the peptidoglycan recognition protein SA (PGRP-SA). A mutation termed osiris disrupts the Gram-negative binding protein 1 (GNBP1) gene and leads to compromised survival of mutant flies after Gram-positive infections, but not after fungal or Gram-negative bacterial challenge. Our results demonstrate that GNBP1 and PGRP-SA can jointly activate the Toll pathway. The potential for a combination of distinct proteins to mediate detection of infectious nonself in the fly will refine the concept of pattern recognition in insects.

1 Unité Propre de Recherche 9022 du CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue René Descartes, F67084 Strasbourg Cedex, France.
2 Exelixis Inc., South San Francisco, CA 94083, USA.


* Present address: Department of Immunology, IMM-30, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

{dagger} To whom correspondence should be addressed. E-mail: D.Ferrandon{at}ibmc.u-strasbg.fr

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Science. ISSN 0036-8075 (print), 1095-9203 (online)