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Originally published in Science Express on 6 November 2003
Science 19 December 2003:
Vol. 302. no. 5653, pp. 2120 - 2124
DOI: 10.1126/science.1091936
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Reports

Separase Regulates INCENP-Aurora B Anaphase Spindle Function Through Cdc14

Gislene Pereira1,2 and Elmar Schiebel1*

The inner centromere-like protein (INCENP) forms a complex with the evolutionarily conserved family of Aurora Bkinases. The INCENP-Aurora complex helps coordinate chromosome segregation, spindle behavior, and cytokinesis during mitosis. INCENP-Aurora associates with kinetochores in metaphase and with spindle microtubules in anaphase, yet the trigger for this abrupt transfer is unknown. Here we show that the conserved phosphatase Cdc14 regulated the yeast INCENP-Aurora complex, Sli15-Ipl1. Cdc14 dephosphorylated Sli15 and thereby directed the complex to spindles. Activation of Cdc14 by separase was sufficient for Sli15 dephosphorylation and relocalization. Cdc14 not only regulates mitotic exit but also modulates spindle midzone assembly through Sli15-Ipl1.

1 The Paterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK.
2 School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK.

* To whom correspondence should be addressed. E-mail: eschiebel{at}picr.man.ac.uk

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