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Science 19 December 2003:
Vol. 302. no. 5653, pp. 2115 - 2117
DOI: 10.1126/science.1091265
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Reports

Maize Genome Sequencing by Methylation Filtration

Lance E. Palmer,* Pablo D. Rabinowicz,* Andrew L. O'Shaughnessy, Vivekanand S. Balija, Lidia U. Nascimento, Sujit Dike, Melissa de la Bastide, Robert A. Martienssen,{dagger} W. Richard McCombie{dagger}

Gene enrichment strategies offer an alternative to sequencing large and repetitive genomes such as that of maize. We report the generation and analysis of nearly 100,000 undermethylated (or methylation filtration) maize sequences. Comparison with the rice genome reveals that methylation filtration results in a more comprehensive representation of maize genes than those that result from expressed sequence tags or transposon insertion sites sequences. About 7% of the repetitive DNA is unmethylated and thus selected in our libraries, but potentially active transposons and unmethylated organelle genomes can be identified. Reverse transcription polymerase chain reaction can be used to finish the maize transcriptome.

Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.


* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: martiens{at}cshl.org (R.A.M.); mccombie{at}cshl.edu (W.R.M.)

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Science. ISSN 0036-8075 (print), 1095-9203 (online)