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Individual cellular clocks in the suprachiasmatic nucleus (SCN),the circadian center, are integrated into a stable and robustpacemaker with a period length of about 24 hours. We used real-timeanalysis of gene expression to show synchronized rhythms ofclock gene transcription across hundreds of neurons within themammalian SCN in organotypic slice culture. Differentially phasedneuronal clocks are topographically arranged across the SCN.A protein synthesis inhibitor set all cell clocks to the sameinitial phase and, after withdrawal, intrinsic interactionsamong cell clocks reestablished the stable program of gene expressionacross the assemblage. Na+-dependent action potentials contributedto establishing cellular synchrony and maintaining spontaneousoscillation across the SCN.
1 Division of Molecular Brain Science, Department of Brain Sciences, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan. 2 Department of Physics, Informatics, and Biology, Yamaguchi University, Yamaguchi 753-8512, Japan. 3 Department of Electronics, Tohoku Institute of Technology, Sendai 982-8577, Japan.
* To whom correspondence should be addressed. E-mail: okamurah{at}kobe-u.ac.jp
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