Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 21 November 2003:
Vol. 302. no. 5649, pp. 1405 - 1407
DOI: 10.1126/science.1089129
Prev | Table of Contents | Next

Reports

Retraction in Amoeboid Cell Motility Powered by Cytoskeletal Dynamics

Long Miao,1* Orion Vanderlinde,1* Murray Stewart,2 Thomas M. Roberts1{dagger}

Cells crawl by coupling protrusion of their leading edge with retraction of their cell body. Protrusion is generated by the polymerization and bundling of filaments, but the mechanism of retraction is less clear. We have reconstituted retraction in vitro by adding Yersinia tyrosine phosphatase to the major sperm protein–based motility apparatus assembled from Ascaris sperm extracts. Retraction in vitro parallels that observed in vivo and is generated primarily by disassembly and rearrangement of the cytoskeleton. Therefore, cytoskeletal dynamics alone, unassisted by conventional motors, are able to generate both of these central components of amoeboid locomotion.

1 Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
2 MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.



* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: roberts{at}bio.fsu.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Exclusion of a Proton ATPase from the Apical Membrane Is Associated with Cell Polarity and Tip Growth in Nicotiana tabacum Pollen Tubes.
A. C. Certal, R. B. Almeida, L. M. Carvalho, E. Wong, N. Moreno, E. Michard, J. Carneiro, J. Rodriguez-Leon, H.-M. Wu, A. Y. Cheung, et al. (2008)
PLANT CELL 20, 614-634
   Abstract »    Full Text »    PDF »
A Ser/Thr Kinase Required for Membrane-associated Assembly of the Major Sperm Protein Motility Apparatus in the Amoeboid Sperm of Ascaris.
K. Yi, S. M. Buttery, M. Stewart, and T. M. Roberts (2007)
Mol. Biol. Cell 18, 1816-1825
   Abstract »    Full Text »    PDF »
Suitable Materials for Soft Tissue Reconstruction: In Vitro Studies of Cell - Triblock Copolymer Interactions.
M. Mattioli-Belmonte, G. Biagini, G. Lucarini, L. Virgili, F. Gabbanelli, S. Amati, F. Cecchet, A.-C. Albertsson, A. Finne, and N. Andronova (2005)
Journal of Bioactive and Compatible Polymers 20, 509-526
   Abstract »    PDF »
C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes.
M. Kosinski, K. McDonald, J. Schwartz, I. Yamamoto, and D. Greenstein (2005)
Development 132, 3357-3369
   Abstract »    Full Text »    PDF »
Regulation of Cell Motility by Tyrosine Phosphorylated Villin.
A. Tomar, Y. Wang, N. Kumar, S. George, B. Ceacareanu, A. Hassid, K. E. Chapman, A. M. Aryal, C. M. Waters, and S. Khurana (2004)
Mol. Biol. Cell 15, 4807-4817
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)