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Originally published in Science Express on 16 October 2003
Science 7 November 2003:
Vol. 302. no. 5647, pp. 1056 - 1060
DOI: 10.1126/science.1089591
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Reports

Induction of APOBEC3G Ubiquitination and Degradation by an HIV-1 Vif-Cul5-SCF Complex

Xianghui Yu,1,2* Yunkai Yu,1* Bindong Liu,1* Kun Luo,1 Wei Kong,2 Panyong Mao,1 Xiao-Fang Yu1,3{dagger}

Human immunodeficiency virus–1 (HIV-1) Vif is essential for viral evasion of host antiviral factor CEM15/APOBEC3G. We report that Vif interacts with cellular proteins Cul5, elongins B and C, and Rbx1 to form an Skp1-cullin-F-box (SCF)–like complex. The ability of Vif to suppress antiviral activity of APOBEC3G was specifically dependent on Cul5-SCF function, allowing Vif to interact with APOBEC3G and induce its ubiquitination and degradation. A Vif mutant that interacted with APOBEC3G but not with Cul5-SCF was functionally inactive. The Cul5-SCF was also required for Vif function in distantly related simian immunodeficiency virus mac. These results indicate that the conserved Cul5-SCF pathway used by Vif is a potential target for antiviral development.

1 Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
2 Jilin University, Jilin, People's Republic of China.
3 Zhejiang University, Zhejiang, People's Republic of China.



* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: xfyu{at}jhsph.edu

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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
Distinct Patterns of Cytokine Regulation of APOBEC3G Expression and Activity in Primary Lymphocytes, Macrophages, and Dendritic Cells.
K. S. Stopak, Y.-L. Chiu, J. Kropp, R. M. Grant, and W. C. Greene (2007)
J. Biol. Chem. 282, 3539-3546
   Abstract »    Full Text »    PDF »
APOBEC3F Can Inhibit the Accumulation of HIV-1 Reverse Transcription Products in the Absence of Hypermutation: COMPARISONS WITH APOBEC3G.
R. K. Holmes, F. A. Koning, K. N. Bishop, and M. H. Malim (2007)
J. Biol. Chem. 282, 2587-2595
   Abstract »    Full Text »    PDF »
Biochemical Differentiation of APOBEC3F and APOBEC3G Proteins Associated with HIV-1 Life Cycle.
X. Wang, P. T. Dolan, Y. Dang, and Y.-H. Zheng (2007)
J. Biol. Chem. 282, 1585-1594
   Abstract »    Full Text »    PDF »
APOBEC3G Expression Is Restricted to Epithelial Cells of the Proximal Convoluted Tubules and Is Not Expressed in the Glomeruli of Macaques.
M. S. Hill, A. Ruiz, L. M. Gomez, J.-M. Miller, N. E.J. Berman, and E. B. Stephens (2007)
J. Histochem. Cytochem. 55, 63-70
   Abstract »    Full Text »    PDF »
Zinc chelation inhibits HIV Vif activity and liberates antiviral function of the cytidine deaminase APOBEC3G.
Z. Xiao, E. Ehrlich, K. Luo, Y. Xiong, and X.-F. Yu (2007)
FASEB J 21, 217-222
   Abstract »    Full Text »    PDF »
Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions.
I. Paul, J. Cui, and E. L. Maynard (2006)
PNAS 103, 18475-18480
   Abstract »    Full Text »    PDF »
Inhibition of Formula-Primed Reverse Transcription by Human APOBEC3G during Human Immunodeficiency Virus Type 1 Replication.
F. Guo, S. Cen, M. Niu, J. Saadatmand, and L. Kleiman (2006)
J. Virol. 80, 11710-11722
   Abstract »    Full Text »    PDF »
Reversed Functional Organization of Mouse and Human APOBEC3 Cytidine Deaminase Domains.
Y. Hakata and N. R. Landau (2006)
J. Biol. Chem. 281, 36624-36631
   Abstract »    Full Text »    PDF »
Generation of HIV-1 derivatives that productively infect macaque monkey lymphoid cells.
K. Kamada, T. Igarashi, M. A. Martin, B. Khamsri, K. Hatcho, T. Yamashita, M. Fujita, T. Uchiyama, and A. Adachi (2006)
PNAS 103, 16959-16964
   Abstract »    Full Text »    PDF »
Identification of APOBEC3DE as Another Antiretroviral Factor from the Human APOBEC Family.
Y. Dang, X. Wang, W. J. Esselman, and Y.-H. Zheng (2006)
J. Virol. 80, 10522-10533
   Abstract »    Full Text »    PDF »
High-molecular-mass APOBEC3G complexes restrict Alu retrotransposition.
Y.-L. Chiu, H. E. Witkowska, S. C. Hall, M. Santiago, V. B. Soros, C. Esnault, T. Heidmann, and W. C. Greene (2006)
PNAS 103, 15588-15593
   Abstract »    Full Text »    PDF »
STAT1-Independent Cell Type-Specific Regulation of Antiviral APOBEC3G by IFN-{alpha}.
P. T. N. Sarkis, S. Ying, R. Xu, and X.-F. Yu (2006)
J. Immunol. 177, 4530-4540
   Abstract »    Full Text »    PDF »
The Anti-HIV-1 Editing Enzyme APOBEC3G Binds HIV-1 RNA and Messenger RNAs That Shuttle between Polysomes and Stress Granules.
S. L. Kozak, M. Marin, K. M. Rose, C. Bystrom, and D. Kabat (2006)
J. Biol. Chem. 281, 29105-29119
   Abstract »    Full Text »    PDF »
Antiviral Potency of APOBEC Proteins Does Not Correlate with Cytidine Deamination..
K. N. Bishop, R. K. Holmes, and M. H. Malim (2006)
J. Virol. 80, 8450-8458
   Abstract »    Full Text »    PDF »
Population Level Analysis of Human Immunodeficiency Virus Type 1 Hypermutation and Its Relationship with APOBEC3G and vif Genetic Variation..
C. Pace, J. Keller, D. Nolan, I. James, S. Gaudieri, C. Moore, and S. Mallal (2006)
J. Virol. 80, 9259-9269
   Abstract »    Full Text »    PDF »


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