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BioProduction 2008

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Science 3 October 2003:
Vol. 302. no. 5642, pp. 106 - 109
DOI: 10.1126/science.1088074
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Reports

Self-Assembly of Proteins into Designed Networks

Philippe Ringler and Georg E. Schulz*

A C4-symmetric tetrameric aldolase was used to produce a quadratic network consisting of the enzyme as a rigid four-way connector and stiff streptavidin rods as spacers. Each aldolase subunit was furnished with a His6 tag for oriented binding to a planar surface and two tethered biotins for binding streptavidin in an oriented manner. The networks were improved by starting with composite units and also by binding to nickel–nitrilotriacetic acid–lipid monolayers. The mesh was adjustable in 5-nanometer increments. The production of a net with switchable mesh was initiated with the use of a calcium ion–containing ß-helix spacer that denatured on calcium ion depletion.

Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, D-79104 Freiburg im Breisgau, Germany.

* To whom correspondence should be addressed. E-mail: schulz{at}bio.chemie.uni-freiburg.de

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Science. ISSN 0036-8075 (print), 1095-9203 (online)