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Discovery of Gene Function by Expression Profiling of the Malaria Parasite Life Cycle
Karine G. Le Roch,1*Yingyao Zhou,2Peter L. Blair,3Muni Grainger,4J. Kathleen Moch,3J. David Haynes,5Patricia De la Vega,3Anthony A. Holder,4Serge Batalov,2Daniel J. Carucci,3Elizabeth A. Winzeler1,2*
The completion of the genome sequence for Plasmodium falciparum,the species responsible for most malaria human deaths, has thepotential to reveal hundreds of new drug targets and proteinsinvolved in pathogenesis. However, only 35% of the genes codefor proteins with an identifiable function. The absence of routinegenetic tools for studying Plasmodium parasites suggests thatthis number is unlikely to change quickly if conventional serialmethods are used to characterize encoded proteins. Here, weuse a high-density oligonucleotide array to generate expressionprofiles of human and mosquito stages of the malaria parasite'slife cycle. Genes with highly correlated levels and temporalpatterns of expression were often involved in similar functionsor cellular processes.
1 Department of Cell Biology ICND202, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. 2 Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA. 3 Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910–7500, USA. 4 Division of Parasitology, National Institute for Medical Research, London NW7 1AA, UK. 5 Department of Immunology, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
* To whom correspondence should be addressed. E-mail: winzeler{at}scripps.edu (E.A.W.); leroch{at}scripps.edu (K.G.L.)
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Andrew P. Waters (12 September 2003) Science301 (5639), 1487.
[DOI: 10.1126/science.1089799] |Summary »|Full Text »|PDF »
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279, 6934-6942
|Abstract »|Full Text »|PDF »
Full-malaria 2004: an enlarged database for comparative studies of full-length cDNAs of malaria parasites, Plasmodium species.
J. Watanabe, Y. Suzuki, M. Sasaki, and S. Sugano (2004)
Nucleic Acids Res.
32, D334-338
|Abstract »|Full Text »|PDF »
Watching Plasmodium falciparum Change Its Clothes.
(2003)
Journal Watch Infectious Diseases
2003, 2
|Full Text »
35% of the genes code for proteins with an identifiable function. The absence of routine genetic tools for studying Plasmodium parasites suggests that this number is unlikely to change quickly if conventional serial methods are used to characterize encoded proteins. Here, we use a high-density oligonucleotide array to generate expression profiles of human and mosquito stages of the malaria parasite's life cycle. Genes with highly correlated levels and temporal patterns of expression were often involved in similar functions or cellular processes. 
