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Science 29 August 2003:
Vol. 301. no. 5637, pp. 1241 - 1243
DOI: 10.1126/science.1087499
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Reports

Persistence Without Pathology in Phosphoglycan-Deficient Leishmania major

Gerald F. Späth,1* Lon-Fey Lye,1 Hiroaki Segawa,2 David L. Sacks,3 Salvatore J. Turco,2 Stephen M. Beverley1{dagger}

Leishmania infections involve an acute phase of replication within macrophages, typically associated with pathology. After recovery parasites persist for long periods, which can lead to severe disease upon reactivation. Unlike the role of host factors, parasite factors affecting persistence are poorly understood. Leishmania major lacking phosphoglycans (lpg2-) were unable to survive in sand flies and macrophages, but retained the ability to persist indefinitely in the mammalian host without inducing disease. The L. major lpg2- thus provides a platform for probing parasite factors implicated in persistence and its role in disease and immunity.

1 Department of Molecular Microbiology, Washington University Medical School, St. Louis, MO 63110, USA. 2 Department of Biochemistry, University of Kentucky Medical School, Lexington KY 40536, USA. 3 Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA.



* Present address: Department of Medical and Molecular Parasitology, New York University School of Medicine, New York, NY 10010, USA.

{dagger} To whom correspondence should be addressed. E-mail: beverley{at}borcim.wustl.edu

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