Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 22 August 2003:
Vol. 301. no. 5636, pp. 1094 - 1096
DOI: 10.1126/science.1085712
Prev | Table of Contents | Next

Reports

Tracking FACT and the RNA Polymerase II Elongation Complex Through Chromatin in Vivo

Abbie Saunders,1 Janis Werner,1 Erik D. Andrulis,1* Takahiro Nakayama,2 Susumu Hirose,2 Danny Reinberg,3 John T. Lis1{dagger}

RNA polymerase II (Pol II) transcription through nucleosomes is facilitated in vitro by the protein complex FACT (Facilitates Chromatin Transcription). Here we show that FACT is associated with actively transcribed Pol II genes on Drosophila polytene chromosomes. FACT displays kinetics of recruitment and of chromosome tracking in vivo similar to Pol II and elongation factors Spt5 and Spt6. Interestingly, FACT does not colocalize with Pol III–transcribed genes, which are known to undergo nucleosome transfer rather than disassembly in vitro. Our observations are consistent with FACT being restricted to transcription that involves nucleosome disassembly mechanisms.

1 Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14850, USA.
2 Department of Developmental Genetics, National Institute of Genetics, 1111 Yata, Mishima, Shizuokaken 411-8540, Japan.
3 Howard Hughes Medical Institute, Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA.



* Present address: Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106–4960, USA.

{dagger} To whom correspondence should be addressed. E-mail: jtl10{at}cornell.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The chromatin remodeling factor CHD8 interacts with elongating RNA polymerase II and controls expression of the cyclin E2 gene.
M. Rodriguez-Paredes, M. Ceballos-Chavez, M. Esteller, M. Garcia-Dominguez, and J. C. Reyes (2009)
Nucleic Acids Res. 37, 2449-2460
   Abstract »    Full Text »    PDF »
Different Genetic Functions for the Rpd3(L) and Rpd3(S) Complexes Suggest Competition between NuA4 and Rpd3(S).
D. Biswas, S. Takahata, and D. J. Stillman (2008)
Mol. Cell. Biol. 28, 4445-4458
   Abstract »    Full Text »    PDF »
The FACT Spt16 "peptidase" domain is a histone H3-H4 binding module.
T. Stuwe, M. Hothorn, E. Lejeune, V. Rybin, M. Bortfeld, K. Scheffzek, and A. G. Ladurner (2008)
PNAS 105, 8884-8889
   Abstract »    Full Text »    PDF »
Regulation of the transcriptional activity of poised RNA polymerase II by the elongation factor ELL.
E. R. Smith, B. Winter, J. C. Eissenberg, and A. Shilatifard (2008)
PNAS 105, 8575-8579
   Abstract »    Full Text »    PDF »
Proteotoxic stress and inducible chaperone networks in neurodegenerative disease and aging.
R. I. Morimoto (2008)
Genes & Dev. 22, 1427-1438
   Abstract »    Full Text »    PDF »
Evidence of Spatially Varying Selection Acting on Four Chromatin-Remodeling Loci in Drosophila melanogaster.
M. T. Levine and D. J. Begun (2008)
Genetics 179, 475-485
   Abstract »    Full Text »    PDF »
P-TEFb Is Critical for the Maturation of RNA Polymerase II into Productive Elongation In Vivo.
Z. Ni, A. Saunders, N. J. Fuda, J. Yao, J.-R. Suarez, W. W. Webb, and J. T. Lis (2008)
Mol. Cell. Biol. 28, 1161-1170
   Abstract »    Full Text »    PDF »
A Role for Chd1 and Set2 in Negatively Regulating DNA Replication in Saccharomyces cerevisiae.
D. Biswas, S. Takahata, H. Xin, R. Dutta-Biswas, Y. Yu, T. Formosa, and D. J. Stillman (2008)
Genetics 178, 649-659
   Abstract »    Full Text »    PDF »
Chd1 and yFACT Act in Opposition in Regulating Transcription.
D. Biswas, R. Dutta-Biswas, and D. J. Stillman (2007)
Mol. Cell. Biol. 27, 6279-6287
   Abstract »    Full Text »    PDF »
Evidence that the Localization of the Elongation Factor Spt16 Across Transcribed Genes Is Dependent Upon Histone H3 Integrity in Saccharomyces cerevisiae.
A. A. Duina, A. Rufiange, J. Bracey, J. Hall, A. Nourani, and F. Winston (2007)
Genetics 177, 101-112
   Abstract »    Full Text »    PDF »
DSIF contributes to transcriptional activation by DNA-binding activators by preventing pausing during transcription elongation.
W. Zhu, T. Wada, S. Okabe, T. Taneda, Y. Yamaguchi, and H. Handa (2007)
Nucleic Acids Res.
   Abstract »    Full Text »    PDF »
Human SSRP1 Has Spt16-dependent and -independent Roles in Gene Transcription.
Y. Li, S. X. Zeng, I. Landais, and H. Lu (2007)
J. Biol. Chem. 282, 6936-6945
   Abstract »    Full Text »    PDF »
The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export.
S. M. Yoh, H. Cho, L. Pickle, R. M. Evans, and K. A. Jones (2007)
Genes & Dev. 21, 160-174
   Abstract »    Full Text »    PDF »
A Gene-Specific Requirement for FACT during Transcription Is Related to the Chromatin Organization of the Transcribed Region.
S. Jimeno-Gonzalez, F. Gomez-Herreros, P. M. Alepuz, and S. Chavez (2006)
Mol. Cell. Biol. 26, 8710-8721
   Abstract »    Full Text »    PDF »
de FACTo Nucleosome Dynamics.
D. Reinberg and R. J. Sims III (2006)
J. Biol. Chem. 281, 23297-23301
   Abstract »    Full Text »    PDF »
Histone trimethylation and the maintenance of transcriptional ONand OFF states by trxG and PcG proteins.
B. Papp and J. Muller (2006)
Genes & Dev. 20, 2041-2054
   Abstract »    Full Text »    PDF »
The Drosophila Dosage Compensation Complex Binds to Polytene Chromosomes Independently of Developmental Changes in Transcription.
I. V. Kotlikova, O. V. Demakova, V. F. Semeshin, V. V. Shloma, L. V. Boldyreva, M. I. Kuroda, and I. F. Zhimulev (2006)
Genetics 172, 963-974
   Abstract »    Full Text »    PDF »
Drosophila Paf1 Modulates Chromatin Structure at Actively Transcribed Genes.
K. Adelman, W. Wei, M. B. Ardehali, J. Werner, B. Zhu, D. Reinberg, and J. T. Lis (2006)
Mol. Cell. Biol. 26, 250-260
   Abstract »    Full Text »    PDF »
Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation..
J.-Y. Huang, W.-H. Chen, Y.-L. Chang, H.-T. Wang, W.-t. Chuang, and S.-C. Lee (2006)
Nucleic Acids Res. 34, 2398-2407
   Abstract »    Full Text »    PDF »
Occupancy of the Drosophila hsp70 promoter by a subset of basal transcription factors diminishes upon transcriptional activation.
L. A. Lebedeva, E. N. Nabirochkina, M. M. Kurshakova, F. Robert, A. N. Krasnov, M. B. Evgen'ev, J. T. Kadonaga, S. G. Georgieva, and L. Tora (2005)
PNAS 102, 18087-18092
   Abstract »    Full Text »    PDF »
Identification and Characterization of Elf1, a Conserved Transcription Elongation Factor in Saccharomyces cerevisiae.
D. Prather, N. J. Krogan, A. Emili, J. F. Greenblatt, and F. Winston (2005)
Mol. Cell. Biol. 25, 10122-10135
   Abstract »    Full Text »    PDF »
Mutational Analysis of an RNA Polymerase II Elongation Factor in Drosophila melanogaster.
M. A. Gerber, A. Shilatifard, and J. C. Eissenberg (2005)
Mol. Cell. Biol. 25, 7803-7811
   Abstract »    Full Text »    PDF »
The Yeast FACT Complex Has a Role in Transcriptional Initiation.
D. Biswas, Y. Yu, M. Prall, T. Formosa, and D. J. Stillman (2005)
Mol. Cell. Biol. 25, 5812-5822
   Abstract »    Full Text »    PDF »
Transcription-induced Chromatin Remodeling at the c-myc Gene Involves the Local Exchange of Histone H2A.Z.
S. D. Farris, E. D. Rubio, J. J. Moon, W. M. Gombert, B. H. Nelson, and A. Krumm (2005)
J. Biol. Chem. 280, 25298-25303
   Abstract »    Full Text »    PDF »
Linking the ubiquitin-proteasome pathway to chromatin remodeling/modification by nuclear receptors.
H K Kinyamu, J Chen, and T K Archer (2005)
J. Mol. Endocrinol. 34, 281-297
   Abstract »    Full Text »    PDF »
CK2 Phosphorylates SSRP1 and Inhibits Its DNA-binding Activity.
Y. Li, D. M. Keller, J. D. Scott, and H. Lu (2005)
J. Biol. Chem. 280, 11869-11875
   Abstract »    Full Text »    PDF »
Replication-independent core histone dynamics at transcriptionally active loci in vivo.
C. Thiriet and J. J. Hayes (2005)
Genes & Dev. 19, 677-682
   Abstract »    Full Text »    PDF »
Molecular characterization of Drosophila NELF.
C.-H. Wu, C. Lee, R. Fan, M. J. Smith, Y. Yamaguchi, H. Handa, and D. S. Gilmour (2005)
Nucleic Acids Res. 33, 1269-1279
   Abstract »    Full Text »    PDF »
Active chromatin domains are defined by acetylation islands revealed by genome-wide mapping.
T.-Y. Roh, S. Cuddapah, and K. Zhao (2005)
Genes & Dev. 19, 542-552
   Abstract »    Full Text »    PDF »
Regulation of Heat Shock Gene Expression by RNA Polymerase II Elongation Factor, Elongin A.
M. Gerber, K. Tenney, J. W. Conaway, R. C. Conaway, J. C. Eissenberg, and A. Shilatifard (2005)
J. Biol. Chem. 280, 4017-4020
   Abstract »    Full Text »    PDF »
Condensed mitotic chromatin is accessible to transcription factors and chromatin structural proteins.
D. Chen, M. Dundr, C. Wang, A. Leung, A. Lamond, T. Misteli, and S. Huang (2005)
J. Cell Biol. 168, 41-54
   Abstract »    Full Text »    PDF »
Evidence for Eviction and Rapid Deposition of Histones upon Transcriptional Elongation by RNA Polymerase II.
M. A. Schwabish and K. Struhl (2004)
Mol. Cell. Biol. 24, 10111-10117
   Abstract »    Full Text »    PDF »
Domain organization of the yeast histone chaperone FACT: the conserved N-terminal domain of FACT subunit Spt16 mediates recovery from replication stress.
A. F. O'Donnell, N. K. Brewster, J. Kurniawan, L. V. Minard, G. C. Johnston, and R. A. Singer (2004)
Nucleic Acids Res. 32, 5894-5906
   Abstract »    Full Text »    PDF »
Elongation by RNA polymerase II: the short and long of it.
R. J. Sims III, R. Belotserkovskaya, and D. Reinberg (2004)
Genes & Dev. 18, 2437-2468
   Abstract »    Full Text »    PDF »
Attenuation of estrogen receptor {alpha}-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor.
S. E. Aiyar, J.-l. Sun, A. L. Blair, C. A. Moskaluk, Y.-z. Lu, Q.-n. Ye, Y. Yamaguchi, A. Mukherjee, D.-m. Ren, H. Handa, et al. (2004)
Genes & Dev. 18, 2134-2146
   Abstract »    Full Text »    PDF »
Repression of Endogenous Smad7 by Ski.
N. G. Denissova and F. Liu (2004)
J. Biol. Chem. 279, 28143-28148
   Abstract »    Full Text »    PDF »
Functional interactions of RNA-capping enzyme with factors that positively and negatively regulate promoter escape by RNA polymerase II.
S. S. Mandal, C. Chu, T. Wada, H. Handa, A. J. Shatkin, and D. Reinberg (2004)
PNAS 101, 7572-7577
   Abstract »    Full Text »    PDF »
Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro.
M. Endoh, W. Zhu, J. Hasegawa, H. Watanabe, D.-K. Kim, M. Aida, N. Inukai, T. Narita, T. Yamada, A. Furuya, et al. (2004)
Mol. Cell. Biol. 24, 3324-3336
   Abstract »    Full Text »    PDF »
Activating and silencing histone modifications form independent allelic switch regions in the imprinted Gnas gene.
T. Li, T. H. Vu, G. A. Ulaner, Y. Yang, J.-F. Hu, and A. R. Hoffman (2004)
Hum. Mol. Genet. 13, 741-750
   Abstract »    Full Text »    PDF »
Histone H3 variants and modifications on transcribed genes.
J. L. Workman and S. M. Abmayr (2004)
PNAS 101, 1429-1430
   Full Text »    PDF »
Histone Deposition Proteins: Links between the DNA Replication Machinery and Epigenetic Gene Silencing.
A.A. FRANCO and P.D. KAUFMAN (2004)
Cold Spring Harb Symp Quant Biol 69, 201-208
   Abstract »    PDF »
FACT Facilitates Transcription-Dependent Nucleosome Alteration.
R. Belotserkovskaya, S. Oh, V. A. Bondarenko, G. Orphanides, V. M. Studitsky, and D. Reinberg (2003)
Science 301, 1090-1093
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)