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Science 11 July 2003:
Vol. 301. no. 5630, pp. 213 - 215
DOI: 10.1126/science.1084920
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Reports

A B Cell-Based Sensor for Rapid Identification of Pathogens

Todd H. Rider,1* Martha S. Petrovick,1 Frances E. Nargi,1 James D. Harper,1 Eric D. Schwoebel,1 Richard H. Mathews,1 David J. Blanchard,1 Laura T. Bortolin,1 Albert M. Young,1 Jianzhu Chen,2 Mark A. Hollis1

We report the use of genetically engineered cells in a pathogen identification sensor. This sensor uses B lymphocytes that have been engineered to emit light within seconds of exposure to specific bacteria and viruses. We demonstrated rapid screening of relevant samples and identification of a variety of pathogens at very low levels. Because of its speed, sensitivity, and specificity, this pathogen identification technology could prove useful for medical diagnostics, biowarfare defense, food- and water-quality monitoring, and other applications.

1 Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA. 2 Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

* To whom correspondence should be addressed. E-mail: thor{at}ll.mit.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)