Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 6 June 2003:
Vol. 300. no. 5625, pp. 1569 - 1574
DOI: 10.1126/science.1084671
Prev | Table of Contents | Next

Reports

Equatorial Retention of the Contractile Actin Ring by Microtubules During Cytokinesis

Mercedes Pardo* and Paul Nurse

In most eukaryotes cytokinesis is brought about by a contractile actin ring located at the division plane. Here, in fission yeast the actin ring was found to be required to generate late-mitotic microtubular structures located at the division plane, and these in turn maintained the medial position of the actin ring. When these microtubular structures were disrupted, the actin ring migrated away from the cell middle in a membrane traffic–dependent manner, resulting in asymmetrical cell divisions that led to genomic instability. We propose that these microtubular structures contribute to a checkpoint control that retains the equatorial position of the ring when progression through cytokinesis is delayed.

Cell Cycle Laboratory, Cancer Research UK London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.

* To whom correspondence should be addressed. E-mail: mercedes.pardo{at}cancer.org.uk

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A perinuclear actin cap regulates nuclear shape.
S. B. Khatau, C. M. Hale, P. J. Stewart-Hutchinson, M. S. Patel, C. L. Stewart, P. C. Searson, D. Hodzic, and D. Wirtz (2009)
PNAS 106, 19017-19022
   Abstract »    Full Text »    PDF »
Cell Cycle-dependent Roles for the FCH-Domain Protein Cdc15p in Formation of the Actomyosin Ring in Schizosaccharomyces pombe.
V. Wachtler, Y. Huang, J. Karagiannis, and M. K. Balasubramanian (2006)
Mol. Biol. Cell 17, 3254-3266
   Abstract »    Full Text »    PDF »
Isolation of Mutations That Bypass the Requirement of the Septation Initiation Network for Septum Formation and Conidiation in Aspergillus nidulans.
J.-M. Kim, L. Lu, R. Shao, J. Chin, and B. Liu (2006)
Genetics 173, 685-696
   Abstract »    Full Text »    PDF »
Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic {gamma}-tubulin complexes.
H. Masuda, T. Toda, R. Miyamoto, T. Haraguchi, and Y. Hiraoka (2006)
Genes Cells 11, 319-336
   Abstract »    Full Text »    PDF »
Plasma Membrane Organization Is Essential for Balancing Competing Pseudopod- and Uropod-promoting Signals during Neutrophil Polarization and Migration.
S. Bodin and M. D. Welch (2005)
Mol. Biol. Cell 16, 5773-5783
   Abstract »    Full Text »    PDF »
The Novel Fission Yeast Protein Pal1p Interacts with Hip1-related Sla2p/End4p and Is Involved in Cellular Morphogenesis.
W. Ge, T. G. Chew, V. Wachtler, S. N. Naqvi, and M. K. Balasubramanian (2005)
Mol. Biol. Cell 16, 4124-4138
   Abstract »    Full Text »    PDF »
Fission Yeast mto2p Regulates Microtubule Nucleation by the Centrosomin-related Protein mto1p.
I. Samejima, P. C. C. Lourenco, H. A. Snaith, and K. E. Sawin (2005)
Mol. Biol. Cell 16, 3040-3051
   Abstract »    Full Text »    PDF »
Effects of {gamma}-Tubulin Complex Proteins on Microtubule Nucleation and Catastrophe in Fission Yeast.
S. Zimmerman and F. Chang (2005)
Mol. Biol. Cell 16, 2719-2733
   Abstract »    Full Text »    PDF »
Mto2p, a Novel Fission Yeast Protein Required for Cytoplasmic Microtubule Organization and Anchoring of the Cytokinetic Actin Ring.
S. Venkatram, J. L. Jennings, A. Link, and K. L. Gould (2005)
Mol. Biol. Cell 16, 3052-3063
   Abstract »    Full Text »    PDF »
End4/Sla2 is involved in establishment of a new growth zone in Schizosaccharomyces pombe.
S. Castagnetti, R. Behrens, and P. Nurse (2005)
J. Cell Sci. 118, 1843-1850
   Abstract »    Full Text »    PDF »
PI(4,5)P2-dependent microdomain assemblies capture microtubules to promote and control leading edge motility.
T. Golub and P. Caroni (2005)
J. Cell Biol. 169, 151-165
   Abstract »    Full Text »    PDF »
Ase1p Organizes Antiparallel Microtubule Arrays during Interphase and Mitosis in Fission Yeast.
I. Loiodice, J. Staub, T. G. Setty, N.-P. T. Nguyen, A. Paoletti, and P. T. Tran (2005)
Mol. Biol. Cell 16, 1756-1768
   Abstract »    Full Text »    PDF »
The Roles of Fission Yeast Ase1 in Mitotic Cell Division, Meiotic Nuclear Oscillation, and Cytokinesis Checkpoint Signaling.
A. Yamashita, M. Sato, A. Fujita, M. Yamamoto, and T. Toda (2005)
Mol. Biol. Cell 16, 1378-1395
   Abstract »    Full Text »    PDF »
The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe.
M. Mishra, J. Karagiannis, S. Trautmann, H. Wang, D. McCollum, and M. K. Balasubramanian (2004)
J. Cell Sci. 117, 3897-3910
   Abstract »    Full Text »    PDF »
Identification and Characterization of Two Novel Proteins Affecting Fission Yeast {gamma}-tubulin Complex Function.
S. Venkatram, J. J. Tasto, A. Feoktistova, J. L. Jennings, A. J. Link, and K. L. Gould (2004)
Mol. Biol. Cell 15, 2287-2301
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)