Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 30 May 2003:
Vol. 300. no. 5624, pp. 1434 - 1436
DOI: 10.1126/science.1083780
Prev | Table of Contents | Next

Reports

Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo

Daniel R. Larson,1 Warren R. Zipfel,1 Rebecca M. Williams,1 Stephen W. Clark,1 Marcel P. Bruchez,2 Frank W. Wise,1 Watt W. Webb1*

The use of semiconductor nanocrystals (quantum dots) as fluorescent labels for multiphoton microscopy enables multicolor imaging in demanding biological environments such as living tissue. We characterized water-soluble cadmium selenide–zinc sulfide quantum dots for multiphoton imaging in live animals. These fluorescent probes have two-photon action cross sections as high as 47,000 Goeppert-Mayer units, by far the largest of any label used in multiphoton microscopy. We visualized quantum dots dynamically through the skin of living mice, in capillaries hundreds of micrometers deep. We found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.

1 School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
2 Quantum Dot Corporation, Hayward, CA 94545, USA.

* To whom correspondence should be addressed. E-mail: www2{at}cornell.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
State of Academic Knowledge on Toxicity and Biological Fate of Quantum Dots.
J. L. Pelley, A. S. Daar, and M. A. Saner (2009)
Toxicol. Sci. 112, 276-296
   Abstract »    Full Text »    PDF »
Noninvasive imaging of dendritic cell migration into lymph nodes using near-infrared fluorescent semiconductor nanocrystals.
Y.-W. Noh, Y. T. Lim, and B. H. Chung (2008)
FASEB J 22, 3908-3918
   Abstract »    Full Text »    PDF »
NSOM/QD-based nanoscale immunofluorescence imaging of antigen-specific T-cell receptor responses during an in vivo clonal V{gamma}2V{delta}2 T-cell expansion.
Y. Chen, L. Shao, Z. Ali, J. Cai, and Z. W. Chen (2008)
Blood 111, 4220-4232
   Abstract »    Full Text »    PDF »
Imaging Epidermal Growth Factor Receptor Expression In vivo: Pharmacokinetic and Biodistribution Characterization of a Bioconjugated Quantum Dot Nanoprobe.
P. Diagaradjane, J. M. Orenstein-Cardona, N. E. Colon-Casasnovas, A. Deorukhkar, S. Shentu, N. Kuno, D. L. Schwartz, J. G. Gelovani, and S. Krishnan (2008)
Clin. Cancer Res. 14, 731-741
   Abstract »    Full Text »    PDF »
Dual-Function Probe for PET and Near-Infrared Fluorescence Imaging of Tumor Vasculature.
W. Cai, K. Chen, Z.-B. Li, S. S. Gambhir, and X. Chen (2007)
J. Nucl. Med. 48, 1862-1870
   Abstract »    Full Text »    PDF »
Specific and covalent labeling of a membrane protein with organic fluorochromes and quantum dots.
R. Bonasio, C. V. Carman, E. Kim, P. T. Sage, K. R. Love, T. R. Mempel, T. A. Springer, and U. H. von Andrian (2007)
PNAS 104, 14753-14758
   Abstract »    Full Text »    PDF »
Quantum Dots: A Quantum Jump for Molecular Imaging?.
K.-H. Lee (2007)
J. Nucl. Med. 48, 1408-1410
   Full Text »    PDF »
Characterization and application of single fluorescent nanodiamonds as cellular biomarkers.
C.-C. Fu, H.-Y. Lee, K. Chen, T.-S. Lim, H.-Y. Wu, P.-K. Lin, P.-K. Wei, P.-H. Tsao, H.-C. Chang, and W. Fann (2007)
PNAS 104, 727-732
   Abstract »    Full Text »    PDF »
Dynamic imaging of dendritic cell extension into the small bowel lumen in response to epithelial cell TLR engagement.
M. Chieppa, M. Rescigno, A. Y.C. Huang, and R. N. Germain (2006)
J. Exp. Med. 203, 2841-2852
   Abstract »    Full Text »    PDF »
Two-photon vibrational spectroscopy for biosciences based on surface-enhanced hyper-Raman scattering.
J. Kneipp, H. Kneipp, and K. Kneipp (2006)
PNAS 103, 17149-17153
   Abstract »    Full Text »    PDF »
Nanodiagnostics: A New Frontier for Clinical Laboratory Medicine.
H. M.E. Azzazy, M. M.H. Mansour, and S. C. Kazmierczak (2006)
Clin. Chem. 52, 1238-1246
   Abstract »    Full Text »    PDF »
Emerging Imaging Techniques.
E. R. McVeigh (2006)
Circ. Res. 98, 879-886
   Abstract »    Full Text »    PDF »
In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space.
R. G. Thorne and C. Nicholson (2006)
PNAS 103, 5567-5572
   Abstract »    Full Text »    PDF »
Behavioral Profiling of Human Transitional Cell Carcinoma Ex vivo..
C. R. Estrada, M. Salanga, D. R. Bielenberg, W. B. Harrell, D. Zurakowski, X. Zhu, M. R. Palmer, M. R. Freeman, and R. M. Adam (2006)
Cancer Res. 66, 3078-3086
   Abstract »    Full Text »    PDF »
Quantum Dot Applications to Neuroscience: New Tools for Probing Neurons and Glia.
S. Pathak, E. Cao, M. C. Davidson, S. Jin, and G. A. Silva (2006)
J. Neurosci. 26, 1893-1895
   Full Text »    PDF »
Quantitative 3D fluorescence technique for the analysis of en face preparations of arterial walls using quantum dot nanocrystals and two-photon excitation laser scanning microscopy.
D. E. Ferrara, D. Weiss, P. H. Carnell, R. P. Vito, D. Vega, X. Gao, S. Nie, and W. R. Taylor (2006)
Am J Physiol Regulatory Integrative Comp Physiol 290, R114-R123
   Abstract »    Full Text »    PDF »
In vitro and in vivo two-photon luminescence imaging of single gold nanorods.
H. Wang, T. B. Huff, D. A. Zweifel, W. He, P. S. Low, A. Wei, and J.-X. Cheng (2005)
PNAS 102, 15752-15756
   Abstract »    Full Text »    PDF »
Blinking and nonradiant dark fraction of water-soluble quantum dots in aqueous solution.
J. Yao, D. R. Larson, H. D. Vishwasrao, W. R. Zipfel, and W. W. Webb (2005)
PNAS 102, 14284-14289
   Abstract »    Full Text »    PDF »
The Convergence of Synthetic Organic and Polymer Chemistries.
C. J. Hawker and K. L. Wooley (2005)
Science 309, 1200-1205
   Abstract »    Full Text »    PDF »
Targeting quantum dots to surface proteins in living cells with biotin ligase.
M. Howarth, K. Takao, Y. Hayashi, and A. Y. Ting (2005)
PNAS 102, 7583-7588
   Abstract »    Full Text »    PDF »
Nanomedicine: current status and future prospects.
S. M. Moghimi, A. C. Hunter, and J. C. Murray (2005)
FASEB J 19, 311-330
   Abstract »    Full Text »    PDF »
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics.
X. Michalet, F. F. Pinaud, L. A. Bentolila, J. M. Tsay, S. Doose, J. J. Li, G. Sundaresan, A. M. Wu, S. S. Gambhir, and S. Weiss (2005)
Science 307, 538-544
   Abstract »    Full Text »    PDF »
Two-Photon Microscopy of Cells and Tissue.
M. Rubart (2004)
Circ. Res. 95, 1154-1166
   Abstract »    Full Text »    PDF »
Imaging Takes a Quantum Leap.
D. S. Lidke and D. J. Arndt-Jovin (2004)
Physiology 19, 322-325
   Abstract »    Full Text »    PDF »
Imaging inflammation of the pancreatic islets in type 1 diabetes.
M. C. Denis, U. Mahmood, C. Benoist, D. Mathis, and R. Weissleder (2004)
PNAS 101, 12634-12639
   Abstract »    Full Text »    PDF »
Examining Intracellular Organelle Function Using Fluorescent Probes: From Animalcules to Quantum Dots.
D. B. Zorov, E. Kobrinsky, M. Juhaszova, and S. J. Sollott (2004)
Circ. Res. 95, 239-252
   Abstract »    Full Text »    PDF »
In Vivo Multiphoton Microscopy of Deep Brain Tissue.
M. J. Levene, D. A. Dombeck, K. A. Kasischke, R. P. Molloy, and W. W. Webb (2004)
J Neurophysiol 91, 1908-1912
   Abstract »    Full Text »    PDF »
Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking.
M. Dahan, S. Levi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller (2003)
Science 302, 442-445
   Abstract »    Full Text »    PDF »
Single metallic nanoparticle imaging for protein detection in cells.
L. Cognet, C. Tardin, D. Boyer, D. Choquet, P. Tamarat, and B. Lounis (2003)
PNAS 100, 11350-11355
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)