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Science 23 May 2003:
Vol. 300. no. 5623, pp. 1288 - 1291
DOI: 10.1126/science.1082588
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Reports

The Birth of an Alternatively Spliced Exon: 3' Splice-Site Selection in Alu Exons

Galit Lev-Maor,1* Rotem Sorek,1,2* Noam Shomron,1 Gil Ast1{dagger}

Alu repetitive elements can be inserted into mature messenger RNAs via a splicing-mediated process termed exonization. To understand the molecular basis and the regulation of the process of turning intronic Alus into new exons, we compiled and analyzed a data set of human exonized Alus. We revealed a mechanism that governs 3' splice-site selection in these exons during alternative splicing. On the basis of these findings, we identified mutations that activated the exonization of a silent intronic Alu.

1 Department of Human Genetics and Molecular Medicine, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel.
2 Compugen, 72 Pinchas Rosen Street, Tel Aviv 69512, Israel.



* These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: gilast{at}post.tau.ac.il

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