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Science 9 May 2003:
Vol. 300. no. 5621, pp. 976 - 980
DOI: 10.1126/science.1083137
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Reports

Structural Basis of Multiple Drug-Binding Capacity of the AcrB Multidrug Efflux Pump

Edward W. Yu,1 Gerry McDermott,2 Helen I. Zgurskaya,1* Hiroshi Nikaido,1 Daniel E. Koshland, Jr.1{dagger}

Multidrug efflux pumps cause serious problems in cancer chemotherapy and treatment of bacterial infections. Yet high-resolution structures of ligandtransporter complexes have previously been unavailable. We obtained x-ray crystallographic structures of the trimeric AcrB pump from Escherichia coli with four structurally diverse ligands. The structures show that three molecules of ligands bind simultaneously to the extremely large central cavity of 5000 cubic angstroms, primarily by hydrophobic, aromatic stacking and van der Waals interactions. Each ligand uses a slightly different subset of AcrB residues for binding. The bound ligand molecules often interact with each other, stabilizing the binding.

1 Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720–3202, USA.
2 Berkeley Center for Structural Biology, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.



* Present address: Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019, USA.

{dagger} To whom correspondence should be addressed. E-mail: dek{at}uclink4.berkeley.edu

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