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Science 25 April 2003:
Vol. 300. no. 5619, pp. 647 - 650
DOI: 10.1126/science.1084149
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Reports

Requirement of Cks2 for the First Metaphase/Anaphase Transition of Mammalian Meiosis

Charles H. Spruck,1* Maria P. de Miguel,2* Adrian P. L. Smith,1 Aimee Ryan,3{dagger} Paula Stein,4 Richard M. Schultz,4 A. Jeannine Lincoln,2 Peter J. Donovan,2 Steven I. Reed1{ddagger}

We generated mice lacking Cks2, one of two mammalian homologs of the yeast Cdk1-binding proteins, Suc1 and Cks1, and found them to be viable but sterile in both sexes. Sterility is due to failure of both male and female germ cells to progress past the first meiotic metaphase. The chromosomal events up through the end of prophase I are normal in both CKS2–/– males and females, suggesting that the phenotype is due directly to failure to enter anaphase and not a consequence of a checkpoint-mediated metaphase I arrest.

1 Department of Molecular Biology, MB-7, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
2 Kimmel Cancer Center, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA.
3 Department of Medicine, University of California, San Diego, School of Medicine, La Jolla, CA 92093, USA.
4 Department of Biology, University of Pennsylvania, 415 South University Avenue, Philadelphia, PA 19104–6018, USA.



{dagger} Present address: Department of Human Genetics, McGill University, Montreal, QC H3H 1P3, Canada.

* These authors contributedequally to this work.

{ddagger} To whom correspondence should be addressed. E-mail: sreed{at}scripps.edu

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