Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Science Policy Alerts

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 25 April 2003:
Vol. 300. no. 5619, pp. 633 - 636
DOI: 10.1126/science.1081813
Prev | Table of Contents | Next

Reports

Roles of NPM2 in Chromatin and Nucleolar Organization in Oocytes and Embryos

Kathleen H. Burns,1,2* Maria M. Viveiros,4*{dagger} Yongsheng Ren ,1* Pei Wang,1,3 Francesco J. DeMayo,3 Donald E. Frail ,5{ddagger} John J. Eppig,4 Martin M. Matzuk1,2,3§

Upon fertilization, remodeling of condensed maternal and paternal gamete DNA occurs to form the diploid genome. In Xenopus laevis, nucleoplasmin 2 (NPM2) decondenses sperm DNA in vitro. To study chromatin remodeling in vivo, we isolated mammalian NPM2 orthologs. Mouse NPM2 accumulates in oocyte nuclei and persists in preimplantation embryos. Npm2 knockout females have fertility defects owing to failed preimplantation embryo development. Although sperm DNA decondensation proceeds without NPM2, abnormalities are evident in oocyte and early embryonic nuclei. These defects include an absence of coalesced nucleolar structures and loss of heterochromatin and deacetylated histone H3 that normally circumscribe nucleoli in oocytes and early embryos, respectively. Thus, Npm2 is a maternal effect gene critical for nuclear and nucleolar organization and embryonic development.

1 Department of Pathology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
2 Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
3 Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
4 The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.
5 Wyeth Research, 500 Arcola Road, Collegeville, PA 19426, USA.


{dagger} Present address: Department of Animal Biology, Center for Transgenesis and Germ Cell Research, University of Pennsylvania, 382 West Street Road, Kennett Square, PA 19348, USA.

{ddagger} Present address: CNS Discovery Research, Pharmacia Corporation, 301 Henrietta Street, Kalamazoo, MI 49007, USA.

* These authors contributed equally to this work.

§ To whom correspondence should be addressed. E-mail: mmatzuk{at}bcm.tmc.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Maternal depletion of CTCF reveals multiple functions during oocyte and preimplantation embryo development.
L.-B. Wan, H. Pan, S. Hannenhalli, Y. Cheng, J. Ma, A. Fedoriw, V. Lobanenkov, K. E. Latham, R. M. Schultz, and M. S. Bartolomei (2008)
Development 135, 2729-2738
   Abstract »    Full Text »    PDF »
Role for PADI6 and the cytoplasmic lattices in ribosomal storage in oocytes and translational control in the early mouse embryo.
P. Yurttas, A. M. Vitale, R. J. Fitzhenry, L. Cohen-Gould, W. Wu, J. A. Gossen, and S. A. Coonrod (2008)
Development 135, 2627-2636
   Abstract »    Full Text »    PDF »
Contribution of the oocyte nucleus and cytoplasm to the determination of meiotic and developmental competence in mice.
A. Inoue, R. Nakajima, M. Nagata, and F. Aoki (2008)
Hum. Reprod. 23, 1377-1384
   Abstract »    Full Text »    PDF »
What can we learn from gene expression profiling of mouse oocytes?.
T. Hamatani, M. Yamada, H. Akutsu, N. Kuji, Y. Mochimaru, M. Takano, M. Toyoda, K. Miyado, A. Umezawa, and Y. Yoshimura (2008)
Reproduction 135, 581-592
   Abstract »    Full Text »    PDF »
The presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo.
M. Romanato, V. Julianelli, M. Zappi, L. Calvo, and J.C. Calvo (2008)
Hum. Reprod. 23, 1145-1150
   Abstract »    Full Text »    PDF »
Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species.
M. Vallee, K. Aiba, Y. Piao, M.-F. Palin, M. S H Ko, and M.-A. Sirard (2008)
Reproduction 135, 439-448
   Abstract »    Full Text »    PDF »
Maternally derived FILIA-MATER complex localizes asymmetrically in cleavage-stage mouse embryos.
M. Ohsugi, P. Zheng, B. Baibakov, L. Li, and J. Dean (2008)
Development 135, 259-269
   Abstract »    Full Text »    PDF »
The evolutionary emergence of cell type-specific genes inferred from the gene expression analysis of Hydra.
J. S. Hwang, H. Ohyanagi, S. Hayakawa, N. Osato, C. Nishimiya-Fujisawa, K. Ikeo, C. N. David, T. Fujisawa, and T. Gojobori (2007)
PNAS 104, 14735-14740
   Abstract »    Full Text »    PDF »
Analysis of TATA-binding protein 2 (TBP2) and TBP expression suggests different roles for the two proteins in regulation of gene expression during oogenesis and early mouse development.
E. Gazdag, A. Rajkovic, M. E. Torres-Padilla, and L. Tora (2007)
Reproduction 134, 51-62
   Abstract »    Full Text »    PDF »
Analysis of Transcription Factor AP-2 Expression and Function During Mouse Preimplantation Development.
Q. Winger, J. Huang, H. J. Auman, M. Lewandoski, and T. Williams (2006)
Biol Reprod 75, 324-333
   Abstract »    Full Text »    PDF »
Long-Term Evolution and Functional Diversification in the Members of the Nucleophosmin/Nucleoplasmin Family of Nuclear Chaperones.
J. M. Eirin-Lopez, L. J. Frehlick, and J. Ausio (2006)
Genetics 173, 1835-1850
   Abstract »    Full Text »    PDF »
Essential Role of the B23/NPM Core Domain in Regulating ARF Binding and B23 Stability.
T. Enomoto, M. S. Lindstrom, A. Jin, H. Ke, and Y. Zhang (2006)
J. Biol. Chem. 281, 18463-18472
   Abstract »    Full Text »    PDF »
Maternal BRG1 regulates zygotic genome activation in the mouse..
S. J. Bultman, T. C. Gebuhr, H. Pan, P. Svoboda, R. M. Schultz, and T. Magnuson (2006)
Genes & Dev. 20, 1744-1754
   Abstract »    Full Text »    PDF »
Basonuclin: a novel mammalian maternal-effect gene.
J. Ma, F. Zeng, R. M. Schultz, and H. Tseng (2006)
Development 133, 2053-2062
   Abstract »    Full Text »    PDF »
Chromatin Decondensation and Nuclear Reprogramming by Nucleoplasmin.
H. Tamada, N. V. Thuan, P. Reed, D. Nelson, N. Katoku-Kikyo, J. Wudel, T. Wakayama, and N. Kikyo (2006)
Mol. Cell. Biol. 26, 1259-1271
   Abstract »    Full Text »    PDF »
Deconstructing mammalian reproduction: using knockouts to define fertility pathways.
A. Roy and M. M Matzuk (2006)
Reproduction 131, 207-219
   Abstract »    Full Text »    PDF »
Further evidence on the role of heparan sulfate as protamine acceptor during the decondensation of human spermatozoa.
M. Romanato, E. Regueira, M. S. Cameo, C. Baldini, L. Calvo, and J. C. Calvo (2005)
Hum. Reprod. 20, 2784-2789
   Abstract »    Full Text »    PDF »
Identification of Novel and Known Oocyte-Specific Genes Using Complementary DNA Subtraction and Microarray Analysis in Three Different Species.
M. Vallee, C. Gravel, M.-F. Palin, H. Reghenas, P. Stothard, D. S. Wishart, and M.-A. Sirard (2005)
Biol Reprod 73, 63-71
   Abstract »    Full Text »    PDF »
Premature ovarian aging in mice deficient for Gpr3.
C. Ledent, I. Demeestere, D. Blum, J. Petermans, T. Hamalainen, G. Smits, and G. Vassart (2005)
PNAS 102, 8922-8926
   Abstract »    Full Text »    PDF »
Mice Deficient in Oocyte-Specific Oligoadenylate Synthetase-Like Protein OAS1D Display Reduced Fertility.
W. Yan, L. Ma, P. Stein, S. A. Pangas, K. H. Burns, Y. Bai, R. M. Schultz, and M. M. Matzuk (2005)
Mol. Cell. Biol. 25, 4615-4624
   Abstract »    Full Text »    PDF »
Differential reprogramming of somatic cell nuclei after transfer into mouse cleavage stage blastomeres.
S. Eckardt, N A. Leu, S. Kurosaka, and K J. McLaughlin (2005)
Reproduction 129, 547-556
   Abstract »    Full Text »    PDF »
A Complex Interaction of Imprinted and Maternal-Effect Genes Modifies Sex Determination in Odd Sex (Ods) Mice.
C. Poirier, Y. Qin, C. P. Adams, Y. Anaya, J. B. Singer, A. E. Hill, E. S. Lander, J. H. Nadeau, and C. E. Bishop (2004)
Genetics 168, 1557-1562
   Abstract »    Full Text »    PDF »
The CCCH tandem zinc-finger protein Zfp36l2 is crucial for female fertility and early embryonic development.
S. B. V. Ramos, D. J. Stumpo, E. A. Kennington, R. S. Phillips, C. B. Bock, F. Ribeiro-Neto, and P. J. Blackshear (2004)
Development 131, 4883-4893
   Abstract »    Full Text »    PDF »
Generation of a bovine oocyte cDNA library and microarray: resources for identification of genes important for follicular development and early embryogenesis.
J. Yao, X. Ren, J. J. Ireland, P. M. Coussens, T. P. L. Smith, and G. W. Smith (2004)
Physiol Genomics 19, 84-92
   Abstract »    Full Text »    PDF »
Molecular Cues to Implantation.
S. K. Dey, H. Lim, S. K. Das, J. Reese, B. C. Paria, T. Daikoku, and H. Wang (2004)
Endocr. Rev. 25, 341-373
   Abstract »    Full Text »    PDF »
Characterization of the ovarian transcriptome through the use of differential analysis of gene expression methodologies.
J. D. Hennebold (2004)
Hum. Reprod. Update 10, 227-239
   Abstract »    Full Text »    PDF »
Developmental Expression and Subcellular Localization of Mouse MATER, an Oocyte-Specific Protein Essential for Early Development.
Z.-B. Tong, L. Gold, A. De Pol, K. Vanevski, H. Dorward, P. Sena, C. Palumbo, C. A. Bondy, and L. M. Nelson (2004)
Endocrinology 145, 1427-1434
   Abstract »    Full Text »    PDF »
Oogenesin Is a Novel Mouse Protein Expressed in Oocytes and Early Cleavage-Stage Embryos.
N. Minami, A. Aizawa, R. Ihara, M. Miyamoto, A. Ohashi, and H. Imai (2003)
Biol Reprod 69, 1736-1742
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)