Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 11 April 2003:
Vol. 300. no. 5617, pp. 332 - 336
DOI: 10.1126/science.1080585
Prev | Table of Contents | Next

Reports

Role of the Arabidopsis Glucose Sensor HXK1 in Nutrient, Light, and Hormonal Signaling

Brandon Moore,*dagger Li Zhou,* Filip Rolland, Qi Hall, Wan-Hsing Cheng,ddagger Yan-Xia Liu, Ildoo Hwang,§ Tamara Jones, Jen Sheenparallel

Glucose modulates many vital processes in photosynthetic plants. Analyses of Arabidopsis glucose insensitive2 (gin2) mutants define the physiological functions of a specific hexokinase (HXK1) in the plant glucose-signaling network. HXK1 coordinates intrinsic signals with extrinsic light intensity. HXK1 mutants lacking catalytic activity still support various signaling functions in gene expression, cell proliferation, root and inflorescence growth, and leaf expansion and senescence, thus demonstrating the uncoupling of glucose signaling from glucose metabolism. The gin2 mutants are also insensitive to auxin and hypersensitive to cytokinin. Plants use HXK as a glucose sensor to interrelate nutrient, light, and hormone signaling networks for controlling growth and development in response to the changing environment.

Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
*   These authors contributed equally to this work.

dagger    Present address: Department of Genetics and Biochemistry, Clemson University, 100 Jordan Hall, Clemson, SC 29631, USA.

ddagger    Present address: Botany Institute, Academia Sinica, Taipei, Taiwan.

§   Present address: Department of Life Science, Pohang University of Science and Technology, Pohang 790-784, Korea.

parallel    To whom correspondence should be addressed. E-mail: sheen{at}molbio.mgh.harvard.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
An autophagy-associated Atg8 protein is involved in the responses of Arabidopsis seedlings to hormonal controls and abiotic stresses.
S. Slavikova, S. Ufaz, T. Avin-Wittenberg, H. Levanony, and G. Galili (2008)
J. Exp. Bot.
   Abstract »    Full Text »    PDF »
Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.).
M. E. Ghanem, A. Albacete, C. Martinez-Andujar, M. Acosta, R. Romero-Aranda, I. C. Dodd, S. Lutts, and F. Perez-Alfocea (2008)
J. Exp. Bot. 59, 3039-3050
   Abstract »    Full Text »    PDF »
Sugar signalling mediates cluster root formation and phosphorus starvation-induced gene expression in white lupin.
K. Zhou, M. Yamagishi, M. Osaki, and K. Masuda (2008)
J. Exp. Bot. 59, 2749-2756
   Abstract »    Full Text »    PDF »
Is the onset of senescence in leaf cells of intact plants due to low or high sugar levels?.
W. G. van Doorn (2008)
J. Exp. Bot.
   Abstract »    Full Text »    PDF »
Auxin as a Model for the Integration of Hormonal Signal Processing and Transduction.
W.D. Teale, F.A. Ditengou, A.D. Dovzhenko, X. Li, A.M. Molendijk, B. Ruperti, I. Paponov, and K. Palme (2008)
Mol Plant 1, 229-237
   Abstract »    Full Text »    PDF »
Sucrose transport in the phloem: integrating root responses to phosphorus starvation.
J. P. Hammond and P. J. White (2008)
J. Exp. Bot. 59, 93-109
   Abstract »    Full Text »    PDF »
Changes in Photosynthetic Rates and Gene Expression of Leaves during a Source Sink Perturbation in Sugarcane.
A. J. McCormick, M. D. Cramer, and D. A. Watt (2008)
Ann. Bot. 101, 89-102
   Abstract »    Full Text »    PDF »
The role of glucose kinase in carbohydrate utilization and extracellular polysaccharide production in Xanthomonas campestris pathovar campestris.
G.-T. Lu, Z.-J. Yang, F.-Y. Peng, Y.-N. Tan, Y.-Q. Tang, J.-X. Feng, D.-J. Tang, Y.-Q. He, and J.-L. Tang (2007)
Microbiology 153, 4284-4294
   Abstract »    Full Text »    PDF »
A Role for F-Actin in Hexokinase-Mediated Glucose Signaling.
R. Balasubramanian, A. Karve, M. Kandasamy, R. B. Meagher, and B. d. Moore (2007)
Plant Physiology 145, 1423-1434
   Abstract »    Full Text »    PDF »
Interaction of SOS2 with Nucleoside Diphosphate Kinase 2 and Catalases Reveals a Point of Connection between Salt Stress and H2O2 Signaling in Arabidopsis thaliana.
P. E. Verslues, G. Batelli, S. Grillo, F. Agius, Y.-S. Kim, J. Zhu, M. Agarwal, S. Katiyar-Agarwal, and J.-K. Zhu (2007)
Mol. Cell. Biol. 27, 7771-7780
   Abstract »    Full Text »    PDF »
Temporal Analysis of Sucrose-induced Phosphorylation Changes in Plasma Membrane Proteins of Arabidopsis.
T. Niittyla, A. T. Fuglsang, M. G. Palmgren, W. B. Frommer, and W. X. Schulze (2007)
Mol. Cell. Proteomics 6, 1711-1726
   Abstract »    Full Text »    PDF »
{beta}-Cell Mitochondria Exhibit Membrane Potential Heterogeneity That Can Be Altered by Stimulatory or Toxic Fuel Levels.
J. D. Wikstrom, S. M. Katzman, H. Mohamed, G. Twig, S. A. Graf, E. Heart, A. J.A. Molina, B. E. Corkey, L. M. de Vargas, N. N. Danial, et al. (2007)
Diabetes 56, 2569-2578
   Abstract »    Full Text »    PDF »
Distinct modulations of the hexokinase1-mediated glucose response and hexokinase1-independent processes by HYS1/CPR5 in Arabidopsis.
T. Aki, M. Konishi, T. Kikuchi, T. Fujimori, T. Yoneyama, and S. Yanagisawa (2007)
J. Exp. Bot.
   Abstract »    Full Text »    PDF »
The Botrytis cinerea hexokinase, Hxk1, but not the glucokinase, Glk1, is required for normal growth and sugar metabolism, and for pathogenicity on fruits.
O. Rui and M. Hahn (2007)
Microbiology 153, 2791-2802
   Abstract »    Full Text »    PDF »
Signaling from an Altered Cell Wall to the Nucleus Mediates Sugar-Responsive Growth and Development in Arabidopsis thaliana.
Y. Li, C. Smith, F. Corke, L. Zheng, Z. Merali, P. Ryden, P. Derbyshire, K. Waldron, and M. W. Bevan (2007)
PLANT CELL 19, 2500-2515
   Abstract »    Full Text »    PDF »
Ethylene Insensitivity Results in Down-Regulation of Rubisco Expression and Photosynthetic Capacity in Tobacco.
D. Tholen, T. L. Pons, L. A.C.J. Voesenek, and H. Poorter (2007)
Plant Physiology 144, 1305-1315
   Abstract »    Full Text »    PDF »
tie-dyed1 Functions Non-Cell Autonomously to Control Carbohydrate Accumulation in Maize Leaves.
R. F. Baker and D. M. Braun (2007)
Plant Physiology 144, 867-878
   Abstract »    Full Text »    PDF »
Glycosyl Hydrolases of Cell Wall are Induced by Sugar Starvation in Arabidopsis.
E.-J. Lee, Y. Matsumura, K. Soga, T. Hoson, and N. Koizumi (2007)
Plant Cell Physiol. 48, 405-413
   Abstract »    Full Text »    PDF »
Sweet Sensor, Surprising Partners.
J.-G. Chen (2007)
Sci. STKE 2007, pe7
   Abstract »    Full Text »    PDF »
Activity of a Second Trypanosoma brucei Hexokinase Is Controlled by an 18-Amino-Acid C-Terminal Tail.
M. T. Morris, C. DeBruin, Z. Yang, J. W. Chambers, K. S. Smith, and J. C. Morris (2006)
Eukaryot. Cell 5, 2014-2023
   Abstract »    Full Text »    PDF »
Cytokinins inhibit epiphyllous plantlet development on leaves of Bryophyllum (Kalanchoe) marnierianum.
R. G. Kulka (2006)
J. Exp. Bot. 57, 4089-4098
   Abstract »    Full Text »    PDF »
Phytohormone signalling pathways interact with sugars during seed germination and seedling development.
K. Yuan and J. Wysocka-Diller (2006)
J. Exp. Bot. 57, 3359-3367
   Abstract »    Full Text »    PDF »
Mitochondria-Associated Hexokinases Play a Role in the Control of Programmed Cell Death in Nicotiana benthamiana.
M. Kim, J.-H. Lim, C. S. Ahn, K. Park, G. T. Kim, W. T. Kim, and H.-S. Pai (2006)
PLANT CELL 18, 2341-2355
   Abstract »    Full Text »    PDF »
Analysis of the Arabidopsis rsr4-1/pdx1-3 Mutant Reveals the Critical Function of the PDX1 Protein Family in Metabolism, Development, and Vitamin B6 Biosynthesis.
S. Wagner, A. Bernhardt, J. E. Leuendorf, C. Drewke, A. Lytovchenko, N. Mujahed, C. Gurgui, W. B. Frommer, E. Leistner, A. R. Fernie, et al. (2006)
PLANT CELL 18, 1722-1735
   Abstract »    Full Text »    PDF »
The Maize Root Plasma Membrane H+-ATPase is Regulated by a Sugar-induced Transduction Pathway.
L. Camoni, M. Marra, A. Garufi, S. Visconti, and P. Aducci (2006)
Plant Cell Physiol. 47, 743-747
   Abstract »    Full Text »    PDF »
Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control.
M. Nicolai, M.A. Roncato, A.S. Canoy, D. Rouquie, X. Sarda, G. Freyssinet, and C. Robaglia (2006)
Plant Physiology 141, 663-673
   Abstract »    Full Text »    PDF »
WRI1 Is Required for Seed Germination and Seedling Establishment.
A. Cernac, C. Andre, S. Hoffmann-Benning, and C. Benning (2006)
Plant Physiology 141, 745-757
   Abstract »    Full Text »    PDF »
The Plastid Protein THYLAKOID FORMATION1 and the Plasma Membrane G-Protein GPA1 Interact in a Novel Sugar-Signaling Mechanism in Arabidopsis.
J. Huang, J. P. Taylor, J.-G. Chen, J. F. Uhrig, D. J. Schnell, T. Nakagawa, K. L. Korth, and A. M. Jones (2006)
PLANT CELL 18, 1226-1238
   Abstract »    Full Text »    PDF »
A putative sensor kinase, Hik31, is involved in the response of Synechocystis sp. strain PCC 6803 to the presence of glucose..
S. Kahlon, K. Beeri, H. Ohkawa, Y. Hihara, O. Murik, I. Suzuki, T. Ogawa, and A. Kaplan (2006)
Microbiology 152, 647-655
   Abstract »    Full Text »    PDF »
Establishing glucose- and ABA-regulated transcription networks in Arabidopsis by microarray analysis and promoter classification using a Relevance Vector Machine.
Y. Li, K. K. Lee, S. Walsh, C. Smith, S. Hadingham, K. Sorefan, G. Cawley, and M. W. Bevan (2006)
Genome Res. 16, 414-427
   Abstract »    Full Text »    PDF »
Diel patterns of leaf C export and of main shoot growth for Flaveria linearis with altered leaf sucrose-starch partitioning.
E. D. Leonardos, B. J. Micallef, M. C. Micallef, and B. Grodzinski (2006)
J. Exp. Bot. 57, 801-814
   Abstract »    Full Text »    PDF »
Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants.
I. Couee, C. Sulmon, G. Gouesbet, and A. El Amrani (2006)
J. Exp. Bot. 57, 449-459
   Abstract »    Full Text »    PDF »
The role of sugars in integrating environmental signals during the regulation of leaf senescence.
A. Wingler, S. Purdy, J. A. MacLean, and N. Pourtau (2006)
J. Exp. Bot. 57, 391-399
   Abstract »    Full Text »    PDF »
Sucrose-Specific Induction of Anthocyanin Biosynthesis in Arabidopsis Requires the MYB75/PAP1 Gene.
S. Teng, J. Keurentjes, L. Bentsink, M. Koornneef, and S. Smeekens (2005)
Plant Physiology 139, 1840-1852
   Abstract »    Full Text »    PDF »
Modulation of Fructokinase Activity of Potato (Solanum tuberosum) Results in Substantial Shifts in Tuber Metabolism.
H. V. Davies, L. V. T. Shepherd, M. M. Burrell, F. Carrari, E. Urbanczyk-Wochniak, A. Leisse, R. D. Hancock, M. Taylor, R. Viola, H. Ross, et al. (2005)
Plant Cell Physiol. 46, 1103-1115
   Abstract »    Full Text »    PDF »
Chloroplasts as source and target of cellular redox regulation: a discussion on chloroplast redox signals in the context of plant physiology.
M. Baier and K.-J. Dietz (2005)
J. Exp. Bot. 56, 1449-1462
   Abstract »    Full Text »    PDF »
Light, the circadian clock, and sugar perception in the control of lignin biosynthesis.
L. A. Rogers, C. Dubos, I. F. Cullis, C. Surman, M. Poole, J. Willment, S. D. Mansfield, and M. M. Campbell (2005)
J. Exp. Bot. 56, 1651-1663
   Abstract »    Full Text »    PDF »
Interaction between Phosphate-Starvation, Sugar, and Cytokinin Signaling in Arabidopsis and the Roles of Cytokinin Receptors CRE1/AHK4 and AHK3.
J. M. Franco-Zorrilla, A. C. Martin, A. Leyva, and J. Paz-Ares (2005)
Plant Physiology 138, 847-857
   Abstract »    Full Text »    PDF »
Endogenous hormones and expression of senescence-related genes in different senescent types of maize.
P. He, M. Osaki, M. Takebe, T. Shinano, and J. Wasaki (2005)
J. Exp. Bot. 56, 1117-1128
   Abstract »    Full Text »    PDF »
Hemoglobin Receptor in Leishmania Is a Hexokinase Located in the Flagellar Pocket.
G. Krishnamurthy, R. Vikram, S. B. Singh, N. Patel, S. Agarwal, G. Mukhopadhyay, S. K. Basu, and A. Mukhopadhyay (2005)
J. Biol. Chem. 280, 5884-5891
   Abstract »    Full Text »    PDF »
Effect of the energy supply on filamentous growth and development in Physcomitrella patens.
M. Thelander, T. Olsson, and H. Ronne (2005)
J. Exp. Bot. 56, 653-662
   Abstract »    Full Text »    PDF »
The Different Growth Responses of the Arabidopsis thaliana Leaf Blade and the Petiole during Shade Avoidance are Regulated by Photoreceptors and Sugar.
T. Kozuka, G. Horiguchi, G.-T. Kim, M. Ohgishi, T. Sakai, and H. Tsukaya (2005)
Plant Cell Physiol. 46, 213-223
   Abstract »    Full Text »    PDF »
Exploring the Temperature-Stress Metabolome of Arabidopsis.
F. Kaplan, J. Kopka, D. W. Haskell, W. Zhao, K. C. Schiller, N. Gatzke, D. Y. Sung, and C. L. Guy (2004)
Plant Physiology 136, 4159-4168
   Abstract »    Full Text »    PDF »
The Arabidopsis Trehalose-6-P Synthase AtTPS1 Gene Is a Regulator of Glucose, Abscisic Acid, and Stress Signaling.
N. Avonce, B. Leyman, J. O. Mascorro-Gallardo, P. Van Dijck, J. M. Thevelein, and G. Iturriaga (2004)
Plant Physiology 136, 3649-3659
   Abstract »    Full Text »    PDF »
Global Transcription Profiling Reveals Multiple Sugar Signal Transduction Mechanisms in Arabidopsis.
J. Price, A. Laxmi, S. K. St. Martin, and J.-C. Jang (2004)
PLANT CELL 16, 2128-2150
   Abstract »    Full Text »    PDF »
Effect of pH on the Stability and Structure of Yeast Hexokinase A: ACIDIC AMINO ACID RESIDUES IN THE CLEFT REGION ARE CRITICAL FOR THE OPENING AND THE CLOSING OF THE STRUCTURE.
D. P. Kumar, A. Tiwari, and R. Bhat (2004)
J. Biol. Chem. 279, 32093-32099
   Abstract »    Full Text »    PDF »
A Conserved Upstream Open Reading Frame Mediates Sucrose-Induced Repression of Translation.
A. Wiese, N. Elzinga, B. Wobbes, and S. Smeekens (2004)
PLANT CELL 16, 1717-1729
   Abstract »    Full Text »    PDF »
Glucose-induced Expression of Carotenoid Biosynthesis Genes in the Dark Is Mediated by Cytosolic pH in the Cyanobacterium Synechocystis sp. PCC 6803.
J.-Y. Ryu, J. Y. Song, J. M. Lee, S. W. Jeong, W. S. Chow, S.-B. Choi, B. J. Pogson, and Y.-I. Park (2004)
J. Biol. Chem. 279, 25320-25325
   Abstract »    Full Text »    PDF »
Responses of primary and secondary metabolism to sugar accumulation revealed by microarray expression analysis of the Arabidopsis mutant, pho3.
J. C. Lloyd and O. V. Zakhleniuk (2004)
J. Exp. Bot. 55, 1221-1230
   Abstract »    Full Text »    PDF »
Stress Tolerance and Glucose Insensitive Phenotypes in Arabidopsis Overexpressing the CpMYB10 Transcription Factor Gene.
M. A. Villalobos, D. Bartels, and G. Iturriaga (2004)
Plant Physiology 135, 309-324
   Abstract »    Full Text »    PDF »
UDP-Glucose Pyrophosphorylase. An Old Protein with New Tricks.
L. A. Kleczkowski, M. Geisler, I. Ciereszko, and H. Johansson (2004)
Plant Physiology 134, 912-918
   Full Text »    PDF »
Sugar and phytohormone response pathways: navigating a signalling network.
S. I. Gibson (2004)
J. Exp. Bot. 55, 253-264
   Abstract »    Full Text »    PDF »
Characterization of Mutants in Arabidopsis Showing Increased Sugar-Specific Gene Expression, Growth, and Developmental Responses.
M. Baier, G. Hemmann, R. Holman, F. Corke, R. Card, C. Smith, F. Rook, and M. W. Bevan (2004)
Plant Physiology 134, 81-91
   Abstract »    Full Text »    PDF »
A Novel Type of Chloroplast Stromal Hexokinase Is the Major Glucose-phosphorylating Enzyme in the Moss Physcomitrella patens.
T. Olsson, M. Thelander, and H. Ronne (2003)
J. Biol. Chem. 278, 44439-44447
   Abstract »    Full Text »    PDF »
The Bifunctional Role of Hexokinase in Metabolism and Glucose Signaling.
G. N. Harrington and D. R. Bush (2003)
PLANT CELL 15, 2493-2496
   Full Text »    PDF »
Mitotic and Postmitotic Senescence in Plants.
S. Gan (2003)
Sci. Aging Knowl. Environ. 2003, re7-7
   Abstract »    Full Text »    PDF »
Regulation of Root Ion Transporters by Photosynthesis: Functional Importance and Relation with Hexokinase.
L. Lejay, X. Gansel, M. Cerezo, P. Tillard, C. Muller, A. Krapp, N. von Wiren, F. Daniel-Vedele, and A. Gojon (2003)
PLANT CELL 15, 2218-2232
   Abstract »    Full Text »    PDF »
Three Genes That Affect Sugar Sensing (Abscisic Acid Insensitive 4, Abscisic Acid Insensitive 5, and Constitutive Triple Response 1) Are Differentially Regulated by Glucose in Arabidopsis.
A. Arroyo, F. Bossi, R. R. Finkelstein, and P. Leon (2003)
Plant Physiology 133, 231-242
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)