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Since the earliest examination of cellular structures, biologists
have been fascinated by observing cells using light microscopy.The
advent of fluorescent labeling technologies plus the plethoraof
sophisticated light microscope techniques now available makestudying
dynamic processes in living cells almost commonplace.For anyone new to
this area, however, it can be daunting to decidewhich techniques or
equipment to try. Here, we aim to give a briefoverview of the main
approaches to live cell imaging, with somemention of their pros and
cons.
1 Department of Biochemistry, University of
Bristol, School of Medical Sciences, University Walk, Bristol, BS8 1TD,
UK.
2 School of Biological Sciences, Oxford Road,
University of Manchester, Manchester, M13 9PT, UK.
*
To whom correspondence should be addressed. E-mail:
viki.allan{at}man.ac.uk
Daniel Zicha, Ian M. Dobbie, Mark R. Holt, James Monypenny, Daniel Y. H. Soong, Colin Gray, and Graham A. Dunn (4 April 2003) Science300 (5616), 142.
[DOI: 10.1126/science.1082026] |Abstract »|Full Text »|PDF »|Supporting Online Material »
PERSPECTIVES
Paul Martin and Susan M. Parkhurst (4 April 2003) Science300 (5616), 63.
[DOI: 10.1126/science.1084148] |Summary »|Full Text »|PDF »
INTRODUCTION TO SPECIAL ISSUE
Stella M. Hurtley and Laura Helmuth (4 April 2003) Science300 (5616), 75.
[DOI: 10.1126/science.300.5616.75] |Summary »|PDF »
NEWS
Mary Beckman (4 April 2003) Science300 (5616), 76.
[DOI: 10.1126/science.300.5616.76] |Summary »|Full Text »|PDF »
NEWS
Greg Miller (4 April 2003) Science300 (5616), 78.
[DOI: 10.1126/science.300.5616.78] |Summary »|Full Text »|PDF »
NEWS
Caroline Seydel (4 April 2003) Science300 (5616), 80.
[DOI: 10.1126/science.300.5616.80] |Summary »|Full Text »|PDF »
Polysialylation Increases Lateral Diffusion of Neural Cell Adhesion Molecule in the Cell Membrane.
F. Conchonaud, S. Nicolas, M.-C. Amoureux, C. Menager, D. Marguet, P.-F. Lenne, G. Rougon, and V. Matarazzo (2007)
J. Biol. Chem.
282, 26266-26274
|Abstract »|Full Text »|PDF »
Spatially Resolved Fluorescence Correlation Spectroscopy Using a Spinning Disk Confocal Microscope.
D. R. Sisan, R. Arevalo, C. Graves, R. McAllister, and J. S. Urbach (2006)
Biophys. J.
91, 4241-4252
|Abstract »|Full Text »|PDF »
Intravital multiphoton microscopy of dynamic renal processes.
B. A. Molitoris and R. M. Sandoval (2005)
Am J Physiol Renal Physiol
288, F1084-F1089
|Abstract »|Full Text »|PDF »
Optical routing and sensing with nanowire assemblies.
D. J. Sirbuly, M. Law, P. Pauzauskie, H. Yan, A. V. Maslov, K. Knutsen, C.-Z. Ning, R. J. Saykally, and P. Yang (2005)
PNAS
102, 7800-7805
|Abstract »|Full Text »|PDF »
Dynamics of Mitochondria and Mitochondrial Ca2+ near the Plasma Membrane of PC12 Cells: A Study by Multimode Microscopy.
D.-M. YANG, C.-C. LIN, H. Y. LIN, C.-C. HUANG, D. P. TSAI, C.-W. CHI, and L.-S. KAO (2005)
Ann. N.Y. Acad. Sci.
1042, 163-167
|Abstract »|Full Text »|PDF »
Roles of the Proline-rich Domain in SLP-76 Subcellular Localization and T Cell Function.
A. L. Singer, S. C. Bunnell, A. E. Obstfeld, M. S. Jordan, J. N. Wu, P. S. Myung, L. E. Samelson, and G. A. Koretzky (2004)
J. Biol. Chem.
279, 15481-15490
|Abstract »|Full Text »|PDF »
Session 8: Imaging/Tumor Dormancy: Imaging TONA M. GILMER, Department of Oncology Biology, GlaxoSmithKline, Research Triangle Park, North Carolina 27709.
