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Science 4 April 2003:
Vol. 300. no. 5616, pp. 127 - 130
DOI: 10.1126/science.1081798
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Reports

Visualizing tmRNA Entry into a Stalled Ribosome

Mikel Valle,1* Reynald Gillet,2* Sukhjit Kaur,1 Anke Henne,3 V. Ramakrishnan,2dagger Joachim Frank14dagger

Bacterial ribosomes stalled on defective messenger RNAs (mRNAs) are rescued by tmRNA, an ~300-nucleotide-long molecule that functions as both transfer RNA (tRNA) and mRNA. Translation then switches from the defective message to a short open reading frame on tmRNA that tags the defective nascent peptide chain for degradation. However, the mechanism by which tmRNA can enter and move through the ribosome is unknown. We present a cryo-electron microscopy study at ~13 to 15 angstroms of the entry of tmRNA into the ribosome. The structure reveals how tmRNA could move through the ribosome despite its complicated topology and also suggests roles for proteins S1 and SmpB in the function of tmRNA.

1 Howard Hughes Medical Institute, Wadsworth Center, Health Research, Inc., Empire State Plaza, Albany, NY 12201-0509, USA.
2 MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
3 Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Grisebachstrasse 8, 37077 Göttingen, Germany.
4 Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, New York 12201-0509, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: ramak{at}mrc-lmb.cam.ac.uk (V.R.); joachim{at}wadsworth.org (J.F.)

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