Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 14 March 2003:
Vol. 299. no. 5613, pp. 1743 - 1747
DOI: 10.1126/science.1081412
Prev | Table of Contents | Next

Reports

Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor

Aaron F. Straight,1* Amy Cheung,1 John Limouze,2 Irene Chen,3 Nick J. Westwood,4 James R. Sellers,2 Timothy J. Mitchison1

Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. We identified a specific inhibitor of nonmuscle myosin II, blebbistatin, that inhibited contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Using blebbistatin and other drugs, we showed that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis. Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.

1 Department of Cell Biology and Institute of Chemistry and Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA.
2 Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
3 Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
4 School of Chemistry, University of St. Andrews, St. Andrews, Fife, KY16 9AJ, UK.
*   To whom correspondence should be addressed. E-mail: aaron_straight{at}hms.harvard.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Early Effects of Lasonolide A on Pancreatic Cancer Cells.
R. A. Isbrucker, E. A. Guzman, T. P. Pitts, and A. E. Wright (2009)
J. Pharmacol. Exp. Ther. 331, 733-739
   Abstract »    Full Text »    PDF »
Myosin II Recruitment during Cytokinesis Independent of Centralspindlin-mediated Phosphorylation.
J. R. Beach and T. T. Egelhoff (2009)
J. Biol. Chem. 284, 27377-27383
   Abstract »    Full Text »    PDF »
Comparison of regulated passive membrane conductance in action potential-firing fast- and slow-twitch muscle.
T. H. Pedersen, W. A. Macdonald, F. V. de Paoli, I. S. Gurung, and O. B. Nielsen (2009)
J. Gen. Physiol. 134, 323-337
   Abstract »    Full Text »    PDF »
Myosin II is required for interkinetic nuclear migration of neural progenitors.
J. Schenk, M. Wilsch-Brauninger, F. Calegari, and W. B. Huttner (2009)
PNAS 106, 16487-16492
   Abstract »    Full Text »    PDF »
Liquids and Structural Glasses Special Feature: An active biopolymer network controlled by molecular motors.
G. H. Koenderink, Z. Dogic, F. Nakamura, P. M. Bendix, F. C. MacKintosh, J. H. Hartwig, T. P. Stossel, and D. A. Weitz (2009)
PNAS 106, 15192-15197
   Abstract »    Full Text »    PDF »
Drug Effect Unveils Inter-head Cooperativity and Strain-dependent ADP Release in Fast Skeletal Actomyosin.
N. Albet-Torres, M. J. Bloemink, T. Barman, R. Candau, K. Frolander, M. A. Geeves, K. Golker, C. Herrmann, C. Lionne, C. Piperio, et al. (2009)
J. Biol. Chem. 284, 22926-22937
   Abstract »    Full Text »    PDF »
Role of Partitioning-defective 1/Microtubule Affinity-regulating Kinases in the Morphogenetic Activity of Helicobacter pylori CagA.
H. Lu, N. Murata-Kamiya, Y. Saito, and M. Hatakeyama (2009)
J. Biol. Chem. 284, 23024-23036
   Abstract »    Full Text »    PDF »
{beta}- and {gamma}-cytoplasmic actins display distinct distribution and functional diversity.
V. Dugina, I. Zwaenepoel, G. Gabbiani, S. Clement, and C. Chaponnier (2009)
J. Cell Sci. 122, 2980-2988
   Abstract »    Full Text »    PDF »
Extracellular ATP dissociates nonmuscle myosin from P2X7 complex: this dissociation regulates P2X7 pore formation.
B. J. Gu, C. Rathsam, L. Stokes, A. B. McGeachie, and J. S. Wiley (2009)
Am J Physiol Cell Physiol 297, C430-C439
   Abstract »    Full Text »    PDF »
RhoA/Rho Kinase Signaling in the Cumulus Mediates Extracellular Matrix Assembly.
R. Yodoi, S. Tamba, K. Morimoto, E. Segi-Nishida, M. Nishihara, A. Ichikawa, S. Narumiya, and Y. Sugimoto (2009)
Endocrinology 150, 3345-3352
   Abstract »    Full Text »    PDF »
Contractility-dependent actin dynamics in cardiomyocyte sarcomeres.
A. Skwarek-Maruszewska, P. Hotulainen, P. K. Mattila, and P. Lappalainen (2009)
J. Cell Sci. 122, 2119-2126
   Abstract »    Full Text »    PDF »
Myosin II regulates extension, growth and patterning in the mammalian cochlear duct.
N. Yamamoto, T. Okano, X. Ma, R. S. Adelstein, and M. W. Kelley (2009)
Development 136, 1977-1986
   Abstract »    Full Text »    PDF »
Myosin IIA Associates with NK Cell Lytic Granules to Enable Their Interaction with F-Actin and Function at the Immunological Synapse.
K. B. Sanborn, G. D. Rak, S. Y. Maru, K. Demers, A. Difeo, J. A. Martignetti, M. R. Betts, R. Favier, P. P. Banerjee, and J. S. Orange (2009)
J. Immunol. 182, 6969-6984
   Abstract »    Full Text »    PDF »
The collagen receptor DDR1 regulates cell spreading and motility by associating with myosin IIA.
Y. Huang, P. Arora, C. A. McCulloch, and W. F. Vogel (2009)
J. Cell Sci. 122, 1637-1646
   Abstract »    Full Text »    PDF »
Mertk Drives Myosin II Redistribution during Retinal Pigment Epithelial Phagocytosis.
D. J. Strick, W. Feng, and D. Vollrath (2009)
Invest. Ophthalmol. Vis. Sci. 50, 2427-2435
   Abstract »    Full Text »    PDF »
Direct evidence for roles of phosphorylated regulatory light chain of myosin II in furrow ingression during cytokinesis in HeLa cells.
S. Asano, K. Hamao, and H. Hosoya (2009)
Genes Cells 14, 555-568
   Abstract »    Full Text »    PDF »
The Cul3/Klhdc5 E3 Ligase Regulates p60/Katanin and Is Required for Normal Mitosis in Mammalian Cells.
C. M. Cummings, C. A. Bentley, S. A. Perdue, P. W. Baas, and J. D. Singer (2009)
J. Biol. Chem. 284, 11663-11675
   Abstract »    Full Text »    PDF »
How to scaffold the contractile ring for a safe cytokinesis - lessons from Anillin-related proteins.
P. Paolo D'Avino (2009)
J. Cell Sci. 122, 1071-1079
   Abstract »    Full Text »    PDF »
Delineating the signals by which repetitive deformation stimulates intestinal epithelial migration across fibronectin.
C. P. Gayer, L. S. Chaturvedi, S. Wang, B. Alston, T. L. Flanigan, and M. D. Basson (2009)
Am J Physiol Gastrointest Liver Physiol 296, G876-G885
   Abstract »    Full Text »    PDF »
Dynamic release of nuclear RanGTP triggers TPX2-dependent microtubule assembly during the apoptotic execution phase.
D. K. Moss, A. Wilde, and J. D. Lane (2009)
J. Cell Sci. 122, 644-655
   Abstract »    Full Text »    PDF »
Myosin-IIA and ICAM-1 Regulate the Interchange between Two Distinct Modes of T Cell Migration.
J. Jacobelli, F. C. Bennett, P. Pandurangi, A. J. Tooley, and M. F. Krummel (2009)
J. Immunol. 182, 2041-2050
   Abstract »    Full Text »    PDF »
The Role of Actomyosin Contractility in the Formation and Dynamics of Actin Bundles During Fibroblast Spreading.
Y. Senju and H. Miyata (2009)
J. Biochem. 145, 137-150
   Abstract »    Full Text »    PDF »
Non-Muscle Myosin IIA Differentially Regulates Intestinal Epithelial Cell Restitution and Matrix Invasion.
B. A. Babbin, S. Koch, M. Bachar, M.-A. Conti, C. A. Parkos, R. S. Adelstein, A. Nusrat, and A. I. Ivanov (2009)
Am. J. Pathol. 174, 436-448
   Abstract »    Full Text »    PDF »
Dorsal Root Ganglion Neurons React to Semaphorin 3A Application through a Biphasic Response that Requires Multiple Myosin II Isoforms.
J. A. Brown, R. B. Wysolmerski, and P. C. Bridgman (2009)
Mol. Biol. Cell 20, 1167-1179
   Abstract »    Full Text »    PDF »
F-Actin and Myosin II Accelerate Catecholamine Release from Chromaffin Granules.
K. Berberian, A. J. Torres, Q. Fang, K. Kisler, and M. Lindau (2009)
J. Neurosci. 29, 863-870
   Abstract »    Full Text »    PDF »
Calcium- and myosin-dependent changes in troponin structure during activation of heart muscle.
Y.-B. Sun, F. Lou, and M. Irving (2009)
J. Physiol. 587, 155-163
   Abstract »    Full Text »    PDF »
Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed.
M. L. Gardel, B. Sabass, L. Ji, G. Danuser, U. S. Schwarz, and C. M. Waterman (2008)
J. Cell Biol. 183, 999-1005
   Abstract »    Full Text »    PDF »
The C-Terminal Tail Region of Nonmuscle Myosin II Directs Isoform-specific Distribution in Migrating Cells.
J. C. Sandquist and A. R. Means (2008)
Mol. Biol. Cell 19, 5156-5167
   Abstract »    Full Text »    PDF »
Modes and Mishaps of Neuronal Migration in the Mammalian Brain.
C. Metin, R. B. Vallee, P. Rakic, and P. G. Bhide (2008)
J. Neurosci. 28, 11746-11752
   Abstract »    Full Text »    PDF »
The Myosin II ATPase Inhibitor Blebbistatin Prevents Thrombin-Induced Inhibition of Intercellular Calcium Wave Propagation in Corneal Endothelial Cells.
R. Ponsaerts, C. D'hondt, G. Bultynck, S. P. Srinivas, J. Vereecke, and B. Himpens (2008)
Invest. Ophthalmol. Vis. Sci. 49, 4816-4827
   Abstract »    Full Text »    PDF »
Retrograde Flow and Myosin II Activity within the Leading Cell Edge Deliver F-Actin to the Lamella to Seed the Formation of Graded Polarity Actomyosin II Filament Bundles in Migrating Fibroblasts.
T. W. Anderson, A. N. Vaughan, and L. P. Cramer (2008)
Mol. Biol. Cell 19, 5006-5018
   Abstract »    Full Text »    PDF »
Tektin 2 is required for central spindle microtubule organization and the completion of cytokinesis.
T. M. Durcan, E. S. Halpin, T. Rao, N. S. Collins, E. K. Tribble, J. E. Hornick, and E. H. Hinchcliffe (2008)
J. Cell Biol. 181, 595-603
   Abstract »    Full Text »    PDF »
Dynein drives nuclear rotation during forward progression of motile fibroblasts.
J. R. Levy and E. L. F. Holzbaur (2008)
J. Cell Sci. 121, 3187-3195
   Abstract »    Full Text »    PDF »
Nonmuscle myosin II is responsible for maintaining endothelial cell basal tone and stress fiber integrity.
Z. M. Goeckeler, P. C. Bridgman, and R. B. Wysolmerski (2008)
Am J Physiol Cell Physiol 295, C994-C1006
   Abstract »    Full Text »    PDF »
Myosin II has distinct functions in PNS and CNS myelin sheath formation.
H. Wang, A. Tewari, S. Einheber, J. L. Salzer, and C. V. Melendez-Vasquez (2008)
J. Cell Biol. 182, 1171-1184
   Abstract »    Full Text »    PDF »
Propofol causes neurite retraction in neurones.
D. Turina, V. M. Loitto, K. Bjornstrom, T. Sundqvist, and C. Eintrei (2008)
Br. J. Anaesth. 101, 374-379
   Abstract »    Full Text »    PDF »
Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner.
H. Hirata, H. Tatsumi, and M. Sokabe (2008)
J. Cell Sci. 121, 2795-2804
   Abstract »    Full Text »    PDF »
aPKC enables development of zonula adherens by antagonizing centripetal contraction of the circumferential actomyosin cables.
M. Kishikawa, A. Suzuki, and S. Ohno (2008)
J. Cell Sci. 121, 2481-2492
   Abstract »    Full Text »    PDF »
Dual role for microtubules in regulating cortical contractility during cytokinesis.
K. Murthy and P. Wadsworth (2008)
J. Cell Sci. 121, 2350-2359
   Abstract »    Full Text »    PDF »
Roles of basolateral solute uptake via NKCC1 and of myosin II in vasopressin-induced cell swelling in inner medullary collecting duct.
C.-L. Chou, M.-J. Yu, E. M. Kassai, R. G. Morris, J. D. Hoffert, S. M. Wall, and M. A. Knepper (2008)
Am J Physiol Renal Physiol 295, F192-F201
   Abstract »    Full Text »    PDF »
Dynamic Movement of the Calcium Sensor STIM1 and the Calcium Channel Orai1 in Activated T-Cells: Puncta and Distal Caps.
V. A. Barr, K. M. Bernot, S. Srikanth, Y. Gwack, L. Balagopalan, C. K. Regan, D. J. Helman, C. L. Sommers, M. Oh-hora, A. Rao, et al. (2008)
Mol. Biol. Cell 19, 2802-2817
   Abstract »    Full Text »    PDF »
Pak1 and Pak2 Mediate Tumor Cell Invasion through Distinct Signaling Mechanisms.
S. J. Coniglio, S. Zavarella, and M. H. Symons (2008)
Mol. Cell. Biol. 28, 4162-4172
   Abstract »    Full Text »    PDF »
Myosin II regulates the shape of three-dimensional intestinal epithelial cysts.
A. I. Ivanov, A. M. Hopkins, G. T. Brown, K. Gerner-Smidt, B. A. Babbin, C. A. Parkos, and A. Nusrat (2008)
J. Cell Sci. 121, 1803-1814
   Abstract »    Full Text »    PDF »
Blebbistatin inhibits the chemotaxis of vascular smooth muscle cells by disrupting the myosin II-actin interaction.
H. H. Wang, H. Tanaka, X. Qin, T. Zhao, L.-H. Ye, T. Okagaki, T. Katayama, A. Nakamura, R. Ishikawa, S. E. Thatcher, et al. (2008)
Am J Physiol Heart Circ Physiol 294, H2060-H2068
   Abstract »    Full Text »    PDF »
mDia2 Induces the Actin Scaffold for the Contractile Ring and Stabilizes Its Position during Cytokinesis in NIH 3T3 Cells.
S. Watanabe, Y. Ando, S. Yasuda, H. Hosoya, N. Watanabe, T. Ishizaki, and S. Narumiya (2008)
Mol. Biol. Cell 19, 2328-2338
   Abstract »    Full Text »    PDF »
Cell polarization during monopolar cytokinesis.
C.-K. Hu, M. Coughlin, C. M. Field, and T. J. Mitchison (2008)
J. Cell Biol. 181, 195-202
   Abstract »    Full Text »    PDF »
PGE2-mediated podosome loss in dendritic cells is dependent on actomyosin contraction downstream of the RhoA-Rho-kinase axis.
S. F. G. van Helden, M. M. Oud, B. Joosten, N. Peterse, C. G. Figdor, and F. N. van Leeuwen (2008)
J. Cell Sci. 121, 1096-1106
   Abstract »    Full Text »    PDF »
Inhibition of "self" engulfment through deactivation of myosin-II at the phagocytic synapse between human cells.
R. K. Tsai and D. E. Discher (2008)
J. Cell Biol. 180, 989-1003
   Abstract »    Full Text »    PDF »
Rho-dependent control of anillin behavior during cytokinesis.
G. R.X. Hickson and P. H. O'Farrell (2008)
J. Cell Biol. 180, 285-294
   Abstract »    Full Text »    PDF »
Nonmuscle myosin heavy chain IIA mediates integrin LFA-1 de-adhesion during T lymphocyte migration.
N. A. Morin, P. W. Oakes, Y.-M. Hyun, D. Lee, Y. E. Chin, M. R. King, T. A. Springer, M. Shimaoka, J. X. Tang, J. S. Reichner, et al. (2008)
J. Exp. Med. 205, 195-205
   Abstract »    Full Text »    PDF »
Nonmuscle myosin II moves in new directions.
M. A. Conti and R. S. Adelstein (2008)
J. Cell Sci. 121, 11-18
   Abstract »    Full Text »    PDF »
Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells.
G. W. Pearson and T. Hunter (2007)
J. Cell Biol. 179, 1555-1567
   Abstract »    Full Text »    PDF »
Oncogenic H-Ras V12 promotes anchorage-independent cytokinesis in human fibroblasts.
M. Thullberg, A. Gad, S. Le Guyader, and S. Stromblad (2007)
PNAS 104, 20338-20343
   Abstract »    Full Text »    PDF »
The Phosphorylation of Myosin II at the Ser1 and Ser2 Is Critical for Normal Platelet-derived Growth Factor induced Reorganization of Myosin Filaments.
S. Komatsu and M. Ikebe (2007)
Mol. Biol. Cell 18, 5081-5090
   Abstract »    Full Text »    PDF »
Atrial cardiomyocyte tachycardia alters cardiac fibroblast function: A novel consideration in atrial remodeling.
B. Burstein, X.-Y. Qi, Y.-H. Yeh, A. Calderone, and S. Nattel (2007)
Cardiovasc Res 76, 442-452
   Abstract »    Full Text »    PDF »
SH4-domain-induced plasma membrane dynamization promotes bleb-associated cell motility.
S. Tournaviti, S. Hannemann, S. Terjung, T. M. Kitzing, C. Stegmayer, J. Ritzerfeld, P. Walther, R. Grosse, W. Nickel, and O. T. Fackler (2007)
J. Cell Sci. 120, 3820-3829
   Abstract »    Full Text »    PDF »
Supervillin slows cell spreading by facilitating myosin II activation at the cell periphery.
N. Takizawa, R. Ikebe, M. Ikebe, and E. J. Luna (2007)
J. Cell Sci. 120, 3792-3803
   Abstract »    Full Text »    PDF »
Mammalian diaphanous-related formin Dia1 controls the organization of E-cadherin-mediated cell-cell junctions.
L. Carramusa, C. Ballestrem, Y. Zilberman, and A. D. Bershadsky (2007)
J. Cell Sci. 120, 3870-3882
   Abstract »    Full Text »    PDF »
Megakaryocyte-restricted MYH9 inactivation dramatically affects hemostasis while preserving platelet aggregation and secretion.
C. Leon, A. Eckly, B. Hechler, B. Aleil, M. Freund, C. Ravanat, M. Jourdain, C. Nonne, J. Weber, R. Tiedt, et al. (2007)
Blood 110, 3183-3191
   Abstract »    Full Text »    PDF »
Liver tetraploidization is controlled by a new process of incomplete cytokinesis.
G. Margall-Ducos, S. Celton-Morizur, D. Couton, O. Bregerie, and C. Desdouets (2007)
J. Cell Sci. 120, 3633-3639
   Abstract »    Full Text »    PDF »
Myosin IIA is required for cytolytic granule exocytosis in human NK cells.
M. M. Andzelm, X. Chen, K. Krzewski, J. S. Orange, and J. L. Strominger (2007)
J. Exp. Med. 204, 2285-2291
   Abstract »    Full Text »    PDF »
The Abl-related Gene Tyrosine Kinase Acts through p190RhoGAP to Inhibit Actomyosin Contractility and Regulate Focal Adhesion Dynamics upon Adhesion to Fibronectin.
J. G. Peacock, A. L. Miller, W. D. Bradley, O. C. Rodriguez, D. J. Webb, and A. J. Koleske (2007)
Mol. Biol. Cell 18, 3860-3872
   Abstract »    Full Text »    PDF »
Multiple Myosins Are Required to Coordinate Actin Assembly with Coat Compression during Compensatory Endocytosis.
H.-Y. E. Yu and W. M. Bement (2007)
Mol. Biol. Cell 18, 4096-4105
   Abstract »    Full Text »    PDF »
Actin myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility.
P. T. Yam, C. A. Wilson, L. Ji, B. Hebert, E. L. Barnhart, N. A. Dye, P. W. Wiseman, G. Danuser, and J. A. Theriot (2007)
J. Cell Biol. 178, 1207-1221
   Abstract »    Full Text »    PDF »
Blebbistatin specifically inhibits actin-myosin interaction in mouse cardiac muscle.
Y. Dou, P. Arlock, and A. Arner (2007)
Am J Physiol Cell Physiol 293, C1148-C1153
   Abstract »    Full Text »    PDF »
Forced Unfolding of Proteins Within Cells.
C. P. Johnson, H.-Y. Tang, C. Carag, D. W. Speicher, and D. E. Discher (2007)
Science 317, 663-666
   Abstract »    Full Text »    PDF »
Mechanisms of host cell exit by the intracellular bacterium Chlamydia.
K. Hybiske and R. S. Stephens (2007)
PNAS 104, 11430-11435
   Abstract »    Full Text »    PDF »
The May-Hegglin anomaly gene MYH9 is a negative regulator of platelet biogenesis modulated by the Rho-ROCK pathway.
Z. Chen, O. Naveiras, A. Balduini, A. Mammoto, M. A. Conti, R. S. Adelstein, D. Ingber, G. Q. Daley, and R. A. Shivdasani (2007)
Blood 110, 171-179
   Abstract »    Full Text »    PDF »
Reversine increases the plasticity of lineage-committed mammalian cells.
S. Chen, S. Takanashi, Q. Zhang, W. Xiong, S. Zhu, E. C. Peters, S. Ding, and P. G. Schultz (2007)
PNAS 104, 10482-10487
   Abstract »    Full Text »    PDF »
Par1b Promotes Hepatic-type Lumen Polarity in Madin Darby Canine Kidney Cells via Myosin II- and E-Cadherin-dependent Signaling.
D. Cohen, Y. Tian, and A. Musch (2007)
Mol. Biol. Cell 18, 2203-2215
   Abstract »    Full Text »    PDF »
Myosin-1a powers the sliding of apical membrane along microvillar actin bundles.
R. E. McConnell and M. J. Tyska (2007)
J. Cell Biol. 177, 671-681
   Abstract »    Full Text »    PDF »
Force activates smooth muscle {alpha}-actin promoter activity through the Rho signaling pathway.
X.-H. Zhao, C. Laschinger, P. Arora, K. Szaszi, A. Kapus, and C. A. McCulloch (2007)
J. Cell Sci. 120, 1801-1809
   Abstract »    Full Text »    PDF »
Endosomal recycling controls plasma membrane area during mitosis.
E. Boucrot and T. Kirchhausen (2007)
PNAS 104, 7939-7944
   Abstract »    Full Text »    PDF »
Microtubule function in fibroblast spreading is modulated according to the tension state of cell-matrix interactions.
S. Rhee, H. Jiang, C.-H. Ho, and F. Grinnell (2007)
PNAS 104, 5425-5430
   Abstract »    Full Text »    PDF »
The actin-based motor protein myosin II regulates MHC class II trafficking and BCR-driven antigen presentation.
F. Vascotto, D. Lankar, G. Faure-Andre, P. Vargas, J. Diaz, D. Le Roux, M.-I. Yuseff, J.-B. Sibarita, M. Boes, G. Raposo, et al. (2007)
J. Cell Biol. 176, 1007-1019
   Abstract »    Full Text »    PDF »
Chemical genetics reveals the requirement for Polo-like kinase 1 activity in positioning RhoA and triggering cytokinesis in human cells.
M. E. Burkard, C. L. Randall, S. Larochelle, C. Zhang, K. M. Shokat, R. P. Fisher, and P. V. Jallepalli (2007)
PNAS 104, 4383-4388
   Abstract »    Full Text »    PDF »
Helicobacter pylori CagA Induces AGS Cell Elongation through a Cell Retraction Defect That Is Independent of Cdc42, Rac1, and Arp2/3.
K. M. Bourzac, C. M. Botham, and K. Guillemin (2007)
Infect. Immun. 75, 1203-1213
   Abstract »    Full Text »    PDF »
Two Regions of the Tail Are Necessary for the Isoform-specific Functions of Nonmuscle Myosin IIB.
M. K. Sato, M. Takahashi, and M. Yazawa (2007)
Mol. Biol. Cell 18, 1009-1017
   Abstract »    Full Text »    PDF »
Cell Contact-dependent Regulation of Epithelial-Myofibroblast Transition via the Rho-Rho Kinase-Phospho-Myosin Pathway.
L. Fan, A. Sebe, Z. Peterfi, A. Masszi, A. C.P. Thirone, O. D. Rotstein, H. Nakano, C. A. McCulloch, K. Szaszi, I. Mucsi, et al. (2007)
Mol. Biol. Cell 18, 1083-1097
   Abstract »    Full Text »    PDF »
Potent Inhibition of Arterial Smooth Muscle Tonic Contractions by the Selective Myosin II Inhibitor, Blebbistatin.
T. J. Eddinger, D. P. Meer, A. S. Miner, J. Meehl, A. S. Rovner, and P. H. Ratz (2007)
J. Pharmacol. Exp. Ther. 320, 865-870
   Abstract »    Full Text »    PDF »
Regulation of Myosin II Dynamics by Phosphorylation and Dephosphorylation of Its Light Chain in Epithelial Cells.
T. Watanabe, H. Hosoya, and S. Yonemura (2007)
Mol. Biol. Cell 18, 605-616
   Abstract »    Full Text »    PDF »
Probing the integrin-actin linkage using high-resolution protein velocity mapping.
C. M. Brown, B. Hebert, D. L. Kolin, J. Zareno, L. Whitmore, A. R. Horwitz, and P. W. Wiseman (2006)
J. Cell Sci. 119, 5204-5214
   Abstract »    Full Text »    PDF »
Cracking up: symmetry breaking in cellular systems.
E. Paluch, J. van der Gucht, and C. Sykes (2006)
J. Cell Biol. 175, 687-692
   Abstract »    Full Text »    PDF »
A role for non-muscle myosin II function in furrow maturation in the early zebrafish embryo.
L. E. Urven, T. Yabe, and F. Pelegri (2006)
J. Cell Sci. 119, 4342-4352
   Abstract »    Full Text »    PDF »
Shootin1: a protein involved in the organization of an asymmetric signal for neuronal polarization.
M. Toriyama, T. Shimada, K. B. Kim, M. Mitsuba, E. Nomura, K. Katsuta, Y. Sakumura, P. Roepstorff, and N. Inagaki (2006)
J. Cell Biol. 175, 147-157
   Abstract »    Full Text »    PDF »
The Role of Myosin II Motor Activity in Distributing Myosin Asymmetrically and Coupling Protrusive Activity to Cell Translocation.
J. Kolega (2006)
Mol. Biol. Cell 17, 4435-4445
   Abstract »    Full Text »    PDF »
Small Molecules, Big Players: the National Cancer Institute's Initiative for Chemical Genetics..
N. Tolliday, P. A. Clemons, P. Ferraiolo, A. N. Koehler, T. A. Lewis, X. Li, S. L. Schreiber, D. S. Gerhard, and S. Eliasof (2006)
Cancer Res. 66, 8935-8942
   Abstract »    Full Text »    PDF »
Dissection of amoeboid movement into two mechanically distinct modes.
K. Yoshida and T. Soldati (2006)
J. Cell Sci. 119, 3833-3844
   Abstract »    Full Text »    PDF »
Mechanism of polarized protrusion formation on neuronal precursors migrating in the developing chicken cerebellum.
A. Sakakibara and A. F. Horwitz (2006)
J. Cell Sci. 119, 3583-3592
   Abstract »    Full Text »    PDF »
A Global, Myosin Light Chain Kinase-dependent Increase in Myosin II Contractility Accompanies the Metaphase-Anaphase Transition in Sea Urchin Eggs.
A. Lucero, C. Stack, A. R. Bresnick, and C. B. Shuster (2006)
Mol. Biol. Cell 17, 4093-4104
   Abstract »    Full Text »    PDF »
A Specific Isoform of Nonmuscle Myosin II-C Is Required for Cytokinesis in a Tumor Cell Line.
S. S. Jana, S. Kawamoto, and R. S. Adelstein (2006)
J. Biol. Chem. 281, 24662-24670
   Abstract »    Full Text »    PDF »
RhoA-kinase coordinates F-actin organization and myosin II activity during semaphorin-3A-induced axon retraction..
G. Gallo (2006)
J. Cell Sci. 119, 3413-3423
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)